5HHZ

Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with 6-(3-methylpyrrol-1-yl)-9H-purine

5HHZ の概要

• エントリーDOI: 10.2210/pdb5hhz/pdb
• 関連するPDBエントリー: 4O95 4OAL
• 分子名称: Cytokinin dehydrogenase 4, FLAVIN-ADENINE DINUCLEOTIDE, 6-(3-methyl-1H-pyrrol-1-yl)-9H-purine, ... (4 entities in total)
• 機能のキーワード: flavoenzyme, plant hormone degradation, oxidase/dehydrogenase, rossmann fold, oxidoreductase
• 由来する生物種: Zea mays (Maize)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57264.03

構造登録者

Kopecny, D.,Briozzo, P. (登録日: 2016-01-11, 公開日: 2016-05-04, 最終更新日: 2024-11-06)

主引用文献

Hluska, T.,Dobrev, P.I.,Tarkowska, D.,Frebortova, J.,Zalabak, D.,Kopecny, D.,Plihal, O.,Kokas, F.,Briozzo, P.,Zatloukal, M.,Motyka, V.,Galuszka, P.
Cytokinin metabolism in maize: Novel evidence of cytokinin abundance, interconversions and formation of a new trans-zeatin metabolic product with a weak anticytokinin activity.
Plant Sci., 247:127-137, 2016

PubMed Abstract: Cytokinins (CKs) are an important group of phytohormones. Their tightly regulated and balanced levels are essential for proper cell division and plant organ development. Here we report precise quantification of CK metabolites and other phytohormones in maize reproductive organs in the course of pollination and kernel maturation. A novel enzymatic activity dependent on NADP(+) converting trans-zeatin (tZ) to 6-(3-methylpyrrol-1-yl)purine (MPP) was detected. MPP shows weak anticytokinin properties and inhibition of CK dehydrogenases due to their ability to bind to an active site in the opposite orientation than substrates. Although the physiological significance of tZ side-chain cyclization is not anticipated as the MPP occurrence in maize tissue is very low, properties of the novel CK metabolite indicate its potential for utilization in plant in vitro tissue culture. Furthermore, feeding experiments with different isoprenoid CKs revealed distinct preferences in glycosylation of tZ and cis-zeatin (cZ). While tZ is preferentially glucosylated at the N9 position, cZ forms mainly O-glucosides. Since O-glucosides, in contrast to N9-glucosides, are resistant to irreversible cleavage catalyzed by CK dehydrogenases, the observed preference of maize CK glycosyltransferases to O-glycosylate zeatin in the cis-position might be a reason why cZ derivatives are over-accumulated in different maize tissues and organs.

PubMed: 27095406
DOI: 10.1016/j.plantsci.2016.03.014

実験手法

X-RAY DIFFRACTION (2 Å)