5H7E

Crystal Structure of native drCPDase

5H7E の概要

• エントリーDOI: 10.2210/pdb5h7e/pdb
• 関連するPDBエントリー: 5H7F
• 分子名称: RNA 2',3'-cyclic phosphodiesterase, GLYCEROL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: rna repair, cpdase, 2, 3 -cyclic phosphodiesterase, hydrolase
• 由来する生物種: Deinococcus radiodurans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21117.96

構造登録者

Han, W.,Hua, Y.,Zhao, Y. (登録日: 2016-11-18, 公開日: 2017-09-27, 最終更新日: 2023-11-08)

主引用文献

Han, W.,Cheng, J.,Zhou, C.,Hua, Y.,Zhao, Y.
Crystal structure of the RNA 2',3'-cyclic phosphodiesterase from Deinococcus radiodurans
Acta Crystallogr F Struct Biol Commun, 73:276-280, 2017

PubMed Abstract: 2',3'-Cyclic phosphodiesterase (CPDase) homologues have been found in all domains of life and are involved in diverse RNA and nucleotide metabolisms. The CPDase from Deinococcus radiodurans was crystallized and the crystals diffracted to 1.6 Å resolution, which is the highest resolution currently known for a CPDase structure. Structural comparisons revealed that the enzyme is in an open conformation in the absence of substrate. Nevertheless, the active site is well formed, and the representative motifs interact with sulfate ion, which suggests a conserved catalytic mechanism.

PubMed: 28471359
DOI: 10.1107/S2053230X17004964

実験手法

X-RAY DIFFRACTION (1.6 Å)