5H64
Cryo-EM structure of mTORC1
Summary for 5H64
| Entry DOI | 10.2210/pdb5h64/pdb |
| EMDB information | 6668 |
| Descriptor | Serine/threonine-protein kinase mTOR, Regulatory-associated protein of mTOR, Target of rapamycin complex subunit LST8 (3 entities in total) |
| Functional Keywords | cryo structure mtor complex, transferase |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 6 |
| Total formula weight | 948736.15 |
| Authors | |
| Primary citation | Yang, H.,Wang, J.,Liu, M.,Chen, X.,Huang, M.,Tan, D.,Dong, M.Q.,Wong, C.C.,Wang, J.,Xu, Y.,Wang, H.W. 4.4 angstrom Resolution Cryo-EM structure of human mTOR Complex 1 Protein Cell, 7:878-887, 2016 Cited by PubMed Abstract: Mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) integrates signals from growth factors, cellular energy levels, stress and amino acids to control cell growth and proliferation through regulating translation, autophagy and metabolism. Here we determined the cryo-electron microscopy structure of human mTORC1 at 4.4 Å resolution. The mTORC1 comprises a dimer of heterotrimer (mTOR-Raptor-mLST8) mediated by the mTOR protein. The complex adopts a hollow rhomboid shape with 2-fold symmetry. Notably, mTORC1 shows intrinsic conformational dynamics. Within the complex, the conserved N-terminal caspase-like domain of Raptor faces toward the catalytic cavity of the kinase domain of mTOR. Raptor shows no caspase activity and therefore may bind to TOS motif for substrate recognition. Structural analysis indicates that FKBP12-Rapamycin may generate steric hindrance for substrate entry to the catalytic cavity of mTORC1. The structure provides a basis to understand the assembly of mTORC1 and a framework to characterize the regulatory mechanism of mTORC1 pathway. PubMed: 27909983DOI: 10.1007/s13238-016-0346-6 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.4 Å) |
Structure validation
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