5H5V

Crystal structure of the flagellar cap protein FliD D1-D2-D3 domains from Escherichia coli

5H5V の概要

• エントリーDOI: 10.2210/pdb5h5v/pdb
• 関連するPDBエントリー: 5H5S 5H5W
• 分子名称: Flagellar hook-associated protein 2 (1 entity in total)
• 機能のキーワード: bacterial flagellar cap protein, structural protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Secreted : A0A094VPA5
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 234871.50

構造登録者

Song, W.S.,Cho, S.Y.,Hong, H.J.,Yoon, S.I. (登録日: 2016-11-09, 公開日: 2017-02-22, 最終更新日: 2024-11-06)

主引用文献

Song, W.S.,Cho, S.Y.,Hong, H.J.,Park, S.C.,Yoon, S.I.
Self-Oligomerizing Structure of the Flagellar Cap Protein FliD and Its Implication in Filament Assembly.
J. Mol. Biol., 429:847-857, 2017

PubMed Abstract: FliD is a self-oligomerizing structural protein that caps the growing end of the bacterial flagellar filament. FliD also plays a key role in the flagellar system by continuously adding a new flagellin protein to the tip of the filament. To structurally characterize FliD oligomerization and to provide a FliD-mediated flagellin polymerization mechanism, we have determined the crystal structures of FliD proteins from Escherichia coli and Salmonella enterica serovar Typhimurium (ecFliD and stFliD, respectively). ecFliD consists of three domains (D1, D2, and D3) and forms a hexamer plate of the D2 and D3 domains that resembles a six-pointed star with legs consisting of the D1 domain. In contrast, the D2 and D3 domains of stFliD assemble into a pentamer as a five-pointed star plate. Despite their distinct oligomeric states, ecFliD and stFliD engage a common molecular surface for oligomerization. FliD also features interdomain and intersubunit flexibility, suggesting that FliD reorganizes its domains and adjacent subunits depending on the FliD binding partner. The similarity of the FliD shape to flagellin and the structural dynamics of FliD led us to propose a FliD-catalyzed filament elongation mechanism. In this model, FliD occupies a position in place of a nascent flagellin until the flagellin reaches the growing end of the filament, and then, FliD moves aside to repeat the positional replacement.

PubMed: 28179186
DOI: 10.1016/j.jmb.2017.02.001

実験手法

X-RAY DIFFRACTION (3 Å)