5H4Z

Crystal structure of S202G mutant of human SYT-5 C2A domain

5H4Z の概要

• エントリーDOI: 10.2210/pdb5h4z/pdb
• 関連するPDBエントリー: 5H4Y
• 分子名称: Synaptotagmin-5, CALCIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: metal binding protein, mutant
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass membrane protein : O00445
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34578.76

構造登録者

Qiu, X.,Ge, J.,Yan, X.,Gao, Y.,Teng, M.,Niu, L. (登録日: 2016-11-02, 公開日: 2016-11-30, 最終更新日: 2023-11-08)

主引用文献

Qiu, X.,Ge, J.,Gao, Y.,Teng, M.,Niu, L.
Structural analysis of Ca(2+)-binding pocket of synaptotagmin 5 C2A domain
Int. J. Biol. Macromol., 95:946-953, 2017

PubMed Abstract: Synaptotagmins constitute a family of multifunctional integral membrane proteins found predominantly on vesicles in neural and endocrine tissues. 17 isoforms of synaptotagmin family in mammals have been identified, 7 isoforms among them are known to be able to bind Ca via their C2 domains. This study presents the crystal structure of the first C2 domain (C2A domain) of synaptotagmin 5 complexed with Ca at 1.90Å resolution. Comparison of the Ca-binding pocket of synaptotagmin 5 C2A domain with other synaptotagmin C2 domains demonstrated that a serine residue locating at Ca-binding loop probably responsible to the conformational variation of Ca-binding pocket, and thus impacts the Ca-binding mechanism of C2 domain, which is verified by structural analysis of the serine mutant and Ca-binding assays via isothermal titration calorimetry. Alteration of Ca-binding mechanism might be correlated with different Ca response rates of synaptotagmins, which is the basis of the functions of synaptotagmins in regulating various types of Ca-triggered vesicle-membrane fusion processes.

PubMed: 27793683
DOI: 10.1016/j.ijbiomac.2016.10.083

実験手法

X-RAY DIFFRACTION (3.01 Å)