5H4U

Crystal structure of cellulase from Antarctic springtail, Cryptopygus antarcticus

5H4U の概要

• エントリーDOI: 10.2210/pdb5h4u/pdb
• 分子名称: Endo-beta-1,4-glucanase (2 entities in total)
• 機能のキーワード: cold-active, cellulase, antarctic springtail, cryptopygus antarcticus, hydrolase
• 由来する生物種: Cryptopygus antarcticus (Antarctic springtail)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 73921.62

構造登録者

An, Y.J.,Hong, S.K.,Cha, S.S. (登録日: 2016-11-02, 公開日: 2017-03-22, 最終更新日: 2024-10-16)

主引用文献

Song, J.M.,Hong, S.K.,An, Y.J.,Kang, M.H.,Hong, K.H.,Lee, Y.H.,Cha, S.S.
Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus.
J. Agric. Food Chem., 65:1630-1640, 2017

PubMed Abstract: The CaCel gene from Antarctic springtail Cryptopygus antarcticus codes for a cellulase belonging to the glycosyl hydrolase family 45 (GHF45). Phylogenetic, biochemical, and structural analyses revealed that the CaCel gene product (CaCel) is closely related to fungal GHF45 endo-β-1,4-glucanases. The organization of five introns within the open reading frame of the CaCel gene indicates its endogenous origin in the genome of the species, which suggests the horizontal transfer of the gene from fungi to the springtail. CaCel exhibited optimal activity at pH 3.5, retained 80% of its activity at 0-10 °C, and maintained a half-life of 4 h at 70 °C. Based on the structural comparison between CaCel and a fungal homologue, we deduced the structural basis for the unusual characteristics of CaCel. Under acidic conditions at 50 °C, CaCel was effective to digest the green algae (Ulva pertusa), suggesting that it could be exploited for biofuel production from seaweeds.

PubMed: 28156112
DOI: 10.1021/acs.jafc.6b05037

実験手法

X-RAY DIFFRACTION (2.6 Å)