5H3X

The structure of the N-terminal of the fibronectin/fibrinogen-binding protein from Streptococcus suis (FBPS)

Summary for 5H3X

• Entry DOI: 10.2210/pdb5h3x/pdb
• Related: 5H3W
• Descriptor: Fibronectin/fibrinogen binding protein (2 entities in total)
• Functional Keywords: fibronectin-binding property, cell adhesion
• Biological source: Streptococcus suis
• Total number of polymer chains: 1
• Total formula weight: 30597.45

Authors

MokiMusyoki, A.,Qi, J.,Gao, G.F. (deposition date: 2016-10-27, release date: 2016-11-16, Last modification date: 2024-03-20)

Primary citation

Musyoki, A.M.,Shi, Z.,Xuan, C.,Lu, G.,Qi, J.,Gao, F.,Zheng, B.,Zhang, Q.,Li, Y.,Haywood, J.,Liu, C.,Yan, J.,Shi, Y.,Gao, G.F.
Structural and functional analysis of an anchorless fibronectin-binding protein FBPS from Gram-positive bacterium Streptococcus suis
Proc. Natl. Acad. Sci. U.S.A., 113:13869-13874, 2016

PubMed Abstract: The anchorless fibronectin-binding proteins (FnBPs) are a group of important virulence factors for which the structures are not available and the functions are not well defined. In this study we performed comprehensive studies on a prototypic member of this group: the fibronectin-/fibrinogen-binding protein from Streptococcus suis (FBPS). The structures of the N- and C-terminal halves (FBPS-N and FBPS-C), which together cover the full-length protein in sequence, were solved at a resolution of 2.1 and 2.6 Å, respectively, and each was found to be composed of two domains with unique folds. Furthermore, we have elucidated the organization of these domains by small-angle X-ray scattering. We further showed that the fibronectin-binding site is located in FBPS-C and that FBPS promotes the adherence of S suis to host cells by attaching the bacteria via FBPS-N. Finally, we demonstrated that FBPS functions both as an adhesin, promoting S suis attachment to host cells, and as a bacterial factor, activating signaling pathways via β1 integrin receptors to induce chemokine production.

PubMed: 27834729
DOI: 10.1073/pnas.1608406113

Experimental method

X-RAY DIFFRACTION (2.1 Å)