5H11

Crystal structure of Zebrafish Sec10

Summary for 5H11

• Entry DOI: 10.2210/pdb5h11/pdb
• Descriptor: Uncharacterized protein, BROMIDE ION (3 entities in total)
• Functional Keywords: exocytosis, helical protein, exocyst complex, membrane traffic
• Biological source: Danio rerio (Zebrafish)
• Total number of polymer chains: 1
• Total formula weight: 59148.86

Authors

Chen, J.,Yamagata, A.,Fukai, S. (deposition date: 2016-10-07, release date: 2017-02-01, Last modification date: 2024-03-20)

Primary citation

Chen, J.,Yamagata, A.,Kubota, K.,Sato, Y.,Goto-Ito, S.,Fukai, S.
Crystal structure of Sec10, a subunit of the exocyst complex
Sci Rep, 7:40909-40909, 2017

PubMed Abstract: The exocyst complex is a heterooctameric protein complex composed of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84. This complex plays an essential role in trafficking secretory vesicles to the plasma membrane through its interaction with phosphatidylinositol 4,5-bisphosphate and small GTPases. To date, the near-full-length structural information of each subunit has been limited to Exo70, although the C-terminal half structures of Sec6, Sec15 and Exo84 and the structures of the small GTPase-binding domains of Sec3, Sec5 and Exo84 have been reported. Here, we report the crystal structure of the near-full-length zebrafish Sec10 (zSec10) at 2.73 Å resolution. The structure of zSec10 consists of tandem antiparallel helix bundles that form a straight rod, like helical core regions of other exocyst subunits. This structure provides the first atomic details of Sec10, which may be useful for future functional and structural studies of this subunit and the exocyst complex.

PubMed: 28098232
DOI: 10.1038/srep40909

Experimental method

X-RAY DIFFRACTION (2.732 Å)