5H0Q5H0Q の概要
| エントリーDOI | 10.2210/pdb5h0q/pdb |
| 分子名称 | Lipid binding protein (2 entities in total) |
| 機能のキーワード | sphingomyelin, cholesterol, lipid binding protein |
| 由来する生物種 | Grifola frondosa |
| タンパク質・核酸の鎖数 | 1 |
| 化学式量合計 | 22793.28 |
| 構造登録者 | Makino, A.,Abe, M.,Ishitsuka, R.,Murate, M.,Kishimoto, T.,Sakai, S.,Hullin-Matsuda, F.,Shimada, Y.,Inaba, T.,Miyatake, H.,Tanaka, H.,Kurahashi, A.,Pack, C.G.,Kasai, R.S.,Kubo, S.,Schieber, N.L.,Dohmae, N.,Tochio, N.,Hagiwara, K.,Sasaki, Y.,Aida, Y.,Fujimori, F.,Kigawa, T.,Nishikori, K.,Parton, R.G.,Kusumi, A.,Sako, Y.,Anderluh, G.,Yamashita, M.,Kobayashi, T.,Greimel, P.,Kobayashi, T. (登録日: 2016-10-06, 公開日: 2016-10-19, 最終更新日: 2024-04-03) |
| 主引用文献 | Makino, A.,Abe, M.,Ishitsuka, R.,Murate, M.,Kishimoto, T.,Sakai, S.,Hullin-Matsuda, F.,Shimada, Y.,Inaba, T.,Miyatake, H.,Tanaka, H.,Kurahashi, A.,Pack, C.G.,Kasai, R.S.,Kubo, S.,Schieber, N.L.,Dohmae, N.,Tochio, N.,Hagiwara, K.,Sasaki, Y.,Aida, Y.,Fujimori, F.,Kigawa, T.,Nishibori, K.,Parton, R.G.,Kusumi, A.,Sako, Y.,Anderluh, G.,Yamashita, M.,Kobayashi, T.,Greimel, P.,Kobayashi, T. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C FASEB J., 31:1301-1322, 2017 Cited by PubMed Abstract: We identified a novel, nontoxic mushroom protein that specifically binds to a complex of sphingomyelin (SM), a major sphingolipid in mammalian cells, and cholesterol (Chol). The purified protein, termed nakanori, labeled cell surface domains in an SM- and Chol-dependent manner and decorated specific lipid domains that colocalized with inner leaflet small GTPase H-Ras, but not K-Ras. The use of nakanori as a lipid-domain-specific probe revealed altered distribution and dynamics of SM/Chol on the cell surface of Niemann-Pick type C fibroblasts, possibly explaining some of the disease phenotype. In addition, that nakanori treatment of epithelial cells after influenza virus infection potently inhibited virus release demonstrates the therapeutic value of targeting specific lipid domains for anti-viral treatment.-Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.-G., Kasai, R. S., Kubo, S., Schieber, N. L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton, R. G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. PubMed: 27492925DOI: 10.1096/fj.201500075R 主引用文献が同じPDBエントリー |
| 実験手法 | X-RAY DIFFRACTION (1.501 Å) |
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