5H02

Crystal structure of Methanohalophilus portucalensis glycine sarcosine N-methyltransferase tetramutant (H21G, E23T, E24N, L28S)

Summary for 5H02

DescriptorGlycine sarcosine N-methyltransferase, S-ADENOSYL-L-HOMOCYSTEINE, TRIMETHYL GLYCINE, ... (4 entities in total)
Functional Keywordss-adenosylmethionine-dependent methyltransferases, sam, adomet-mtase, class i monomethylation, glycine, sarcosine, rate-limiting enzyme, betaine biosynthesis, betaine-mediated feedback inhibition, transferase
Biological sourceMethanohalophilus portucalensis FDF-1
Total number of polymer chains1
Total molecular weight33139.62
Authors
Lee, Y.R.,Lin, T.S.,Lai, S.J.,Liu, M.S.,Lai, M.C.,Chan, N.L. (deposition date: 2016-10-02, release date: 2016-11-23, Last modification date: 2017-01-18)
Primary citation
Lee, Y.R.,Lin, T.S.,Lai, S.J.,Liu, M.S.,Lai, M.C.,Chan, N.L.
Structural Analysis of Glycine Sarcosine N-methyltransferase from Methanohalophilus portucalensis Reveals Mechanistic Insights into the Regulation of Methyltransferase Activity.
Sci Rep, 6:38071-38071, 2016
PubMed: 27934872 (PDB entries with the same primary citation)
DOI: 10.1038/srep38071
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.776 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.1962006.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution