5GXG

High-resolution crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin

5GXG の概要

• エントリーDOI: 10.2210/pdb5gxg/pdb
• 分子名称: Camphor 5-monooxygenase, Putidaredoxin, PROTOPORPHYRIN IX CONTAINING FE, ... (7 entities in total)
• 機能のキーワード: inter-protein electron transfer, oxidoreductase-electron transport complex, oxidoreductase/electron transport
• 由来する生物種: Pseudomonas putida; 詳細
• 細胞内の位置: Cytoplasm : P00183
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59709.00

構造登録者

Kikui, Y.,Hiruma, Y.,Ubbink, M.,Nojiri, M. (登録日: 2016-09-17, 公開日: 2017-01-18, 最終更新日: 2023-11-08)

主引用文献

Andraojc, W.,Hiruma, Y.,Liu, W.M.,Ravera, E.,Nojiri, M.,Parigi, G.,Luchinat, C.,Ubbink, M.
Identification of productive and futile encounters in an electron transfer protein complex
Proc. Natl. Acad. Sci. U.S.A., 114:E1840-E1847, 2017

PubMed Abstract: Well-defined, stereospecific states in protein complexes are often in exchange with an ensemble of more dynamic orientations: the encounter states. The structure of the stereospecific complex between cytochrome P450cam and putidaredoxin was solved recently by X-ray diffraction as well as paramagnetic NMR spectroscopy. Other than the stereospecific complex, the NMR data clearly show the presence of additional states in the complex in solution. In these encounter states, populated for a small percentage of the time, putidaredoxin assumes multiple orientations and samples a large part of the surface of cytochrome P450cam. To characterize the nature of the encounter states, an extensive paramagnetic NMR dataset has been analyzed using the Maximum Occurrence of Regions methodology. The analysis reveals the location and maximal spatial extent of the additional states needed to fully explain the NMR data. Under the assumption of sparsity of the size of the conformational ensemble, several minor states can be located quite precisely. The distribution of these minor states correlates with the electrostatic potential map around cytochrome P450cam. Whereas some minor states are on isolated positively charged patches, others are connected to the stereospecific site via positively charged paths. The existence of electrostatically favorable pathways between the stereospecific interaction site and the different minor states or lack thereof suggests a means to discriminate between productive and futile encounter states.

PubMed: 28223532
DOI: 10.1073/pnas.1616813114

実験手法

X-RAY DIFFRACTION (1.7 Å)