5GWE

cytochrome P450 CREJ

5GWE の概要

• エントリーDOI: 10.2210/pdb5gwe/pdb
• 分子名称: Cytochrome P450, PROTOPORPHYRIN IX CONTAINING FE, (4-methylphenyl) dihydrogen phosphate, ... (5 entities in total)
• 機能のキーワード: cytochrome p450, complex, electron transport
• 由来する生物種: Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 186972.99

構造登録者

Dong, S.,liu, X.,Wang, X.,Feng, Y. (登録日: 2016-09-11, 公開日: 2017-07-12, 最終更新日: 2023-11-08)

主引用文献

Du, L.,Dong, S.,Zhang, X.,Jiang, C.,Chen, J.,Yao, L.,Wang, X.,Wan, X.,Liu, X.,Wang, X.,Huang, S.,Cui, Q.,Feng, Y.,Liu, S.J.,Li, S.
Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system
Proc. Natl. Acad. Sci. U.S.A., 114:E5129-E5137, 2017

PubMed Abstract: Selective oxidation of aliphatic C-H bonds in alkylphenols serves significant roles not only in generation of functionalized intermediates that can be used to synthesize diverse downstream chemical products, but also in biological degradation of these environmentally hazardous compounds. Chemo-, regio-, and stereoselectivity; controllability; and environmental impact represent the major challenges for chemical oxidation of alkylphenols. Here, we report the development of a unique chemomimetic biocatalytic system originated from the Gram-positive bacterium The system consisting of CreHI (for installation of a phosphate directing/anchoring group), CreJEF/CreG/CreC (for oxidation of alkylphenols), and CreD (for directing/anchoring group offloading) is able to selectively oxidize the aliphatic C-H bonds of - and -alkylated phenols in a controllable manner. Moreover, the crystal structures of the central P450 biocatalyst CreJ in complex with two representative substrates provide significant structural insights into its substrate flexibility and reaction selectivity.

PubMed: 28607077
DOI: 10.1073/pnas.1702317114

実験手法

X-RAY DIFFRACTION (2 Å)