5GW0

Crystal structure of SNX16 PX-Coiled coil

5GW0 の概要

• エントリーDOI: 10.2210/pdb5gw0/pdb
• 関連するPDBエントリー: 5GW1 5GW8
• 分子名称: Sorting nexin-16 (1 entity in total)
• 機能のキーワード: sorting nexin, px domain, endosome sorting, protein transport
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Early endosome membrane; Peripheral membrane protein; Cytoplasmic side: P57768
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 128706.40

構造登録者

Xu, J.,Liu, J. (登録日: 2016-09-08, 公開日: 2017-09-13, 最終更新日: 2024-10-30)

主引用文献

Xu, J.,Zhang, L.,Ye, Y.,Shan, Y.,Wan, C.,Wang, J.,Pei, D.,Shu, X.,Liu, J.
SNX16 Regulates the Recycling of E-Cadherin through a Unique Mechanism of Coordinated Membrane and Cargo Binding.
Structure, 25:1251-1263.e5, 2017

PubMed Abstract: E-Cadherin is a major component of adherens junctions on cell surfaces. SNX16 is a unique member of sorting nexins that contains a coiled-coil (CC) domain downstream of the PX domain. We report here that SNX16 regulates the recycling trafficking of E-cadherin. We solved the crystal structure of PX-CC unit of SNX16 and revealed a unique shear shaped homodimer. We identified a novel PI3P binding pocket in SNX16 that consists of both the PX and the CC domains. Surprisingly, we showed that the PPII/α2 loop, which is generally regarded as a membrane insertion loop in PX family proteins, is involved in the E-cadherin binding with SNX16. We then proposed a multivalent membrane binding model for SNX16. Our study postulates a new mechanism for coordinated membrane binding and cargo binding for SNX family proteins in general, and provide novel insights into recycling trafficking of E-cadherin.

PubMed: 28712807
DOI: 10.1016/j.str.2017.06.015

実験手法

X-RAY DIFFRACTION (3.3 Å)