5GUY

Structural and functional study of chuY from E. coli CFT073 strain

5GUY の概要

• エントリーDOI: 10.2210/pdb5guy/pdb
• 分子名称: Uncharacterized protein chuY (2 entities in total)
• 機能のキーワード: chuy, flavin reductase, oxidoreductase
• 由来する生物種: Escherichia coli strain CFT073
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46065.34

構造登録者

Kim, H.,Choi, J.,HA, S.C.,Chaurasia, A.K.,Kim, D.,Kim, K.K. (登録日: 2016-08-31, 公開日: 2017-03-08)

主引用文献

Kim, H.,Chaurasia, A.K.,Kim, T.,Choi, J.,Ha, S.C.,Kim, D.,Kim, K.K.
Structural and functional study of ChuY from Escherichia coli strain CFT073
Biochem. Biophys. Res. Commun., 482:1176-1182, 2017

PubMed Abstract: The uropathogenic Escherichia coli strain CFT073 contains multiple iron and heme transport systems, which facilitate infection of the host urinary tract. To elucidate the molecular and cellular function of ChuY, a hypothetical gene in the heme degradation/utilization pathway, we solved the crystal structure of ChuY at 2.4 Å resolution. ChuY has high structural homology with human biliverdin and flavin reductase. We confirmed that ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity. A chuY deletion-insertion strain showed reduced survival potential compared to wild-type and complemented strains in mammalian cells. Current results suggest ChuY acts as a reductase in heme homeostasis to maintain the virulence potential of E. coli CFT073.

PubMed: 27919686
DOI: 10.1016/j.bbrc.2016.12.008

実験手法

X-RAY DIFFRACTION (2.399 Å)