5GUX

Cytochrome c-dependent nitric oxide reductase (cNOR) from Pseudomonas aeruginosa in complex with xenon

5GUX の概要

• エントリーDOI: 10.2210/pdb5gux/pdb
• 関連するPDBエントリー: 3O0R 5GUW
• 分子名称: Antibody fab fragment light chain, CALCIUM ION, HEME C, ... (11 entities in total)
• 機能のキーワード: metal-binding, membrane protein
• 由来する生物種: Mus musculus; 詳細
• 細胞内の位置: Cell membrane ; Multi-pass membrane protein : Q59647; Cell membrane; Single-pass membrane protein: Q59646
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 120295.47

構造登録者

Ishii, S.,Terasaka, E.,Sugimoto, H.,Shiro, Y.,Tosha, T. (登録日: 2016-08-31, 公開日: 2017-08-16, 最終更新日: 2024-11-13)

主引用文献

Terasaka, E.,Yamada, K.,Wang, P.H.,Hosokawa, K.,Yamagiwa, R.,Matsumoto, K.,Ishii, S.,Mori, T.,Yagi, K.,Sawai, H.,Arai, H.,Sugimoto, H.,Sugita, Y.,Shiro, Y.,Tosha, T.
Dynamics of nitric oxide controlled by protein complex in bacterial system.
Proc. Natl. Acad. Sci. U.S.A., 114:9888-9893, 2017

PubMed Abstract: Nitric oxide (NO) plays diverse and significant roles in biological processes despite its cytotoxicity, raising the question of how biological systems control the action of NO to minimize its cytotoxicity in cells. As a great example of such a system, we found a possibility that NO-generating nitrite reductase (NiR) forms a complex with NO-decomposing membrane-integrated NO reductase (NOR) to efficiently capture NO immediately after its production by NiR in anaerobic nitrate respiration called denitrification. The 3.2-Å resolution structure of the complex of one NiR functional homodimer and two NOR molecules provides an idea of how these enzymes interact in cells, while the structure may not reflect the one in cells due to the membrane topology. Subsequent all-atom molecular dynamics (MD) simulations of the enzyme complex model in a membrane and structure-guided mutagenesis suggested that a few interenzyme salt bridges and coulombic interactions of NiR with the membrane could stabilize the complex of one NiR homodimer and one NOR molecule and contribute to rapid NO decomposition in cells. The MD trajectories of the NO diffusion in the NiR:NOR complex with the membrane showed that, as a plausible NO transfer mechanism, NO released from NiR rapidly migrates into the membrane, then binds to NOR. These results help us understand the mechanism of the cellular control of the action of cytotoxic NO.

PubMed: 28847930
DOI: 10.1073/pnas.1621301114

実験手法

X-RAY DIFFRACTION (3.3 Å)