5GUN

Crystal structure of d(GTGGAATGGAAC)

「4RZN」から置き換えられました 「4NZX」から置き換えられました

5GUN の概要

• エントリーDOI: 10.2210/pdb5gun/pdb
• 分子名称: DNA (5'-D(*GP*TP*GP*GP*AP*AP*TP*GP*GP*AP*AP*C)-3'), COBALT (II) ION (3 entities in total)
• 機能のキーワード: (gga) motif, dna expansion in sca31 patient, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 22980.26

構造登録者

Satange, R.B.,Kao, Y.F.,Hou, M.H. (登録日: 2016-08-29, 公開日: 2017-08-30, 最終更新日: 2024-03-20)

主引用文献

Huang, T.Y.,Chang, C.K.,Kao, Y.F.,Chin, C.H.,Ni, C.W.,Hsu, H.Y.,Hu, N.J.,Hsieh, L.C.,Chou, S.H.,Lee, I.R.,Hou, M.H.
Parity-dependent hairpin configurations of repetitive DNA sequence promote slippage associated with DNA expansion
Proc. Natl. Acad. Sci. U.S.A., 114:9535-9540, 2017

PubMed Abstract: Repetitive DNA sequences are ubiquitous in life, and changes in the number of repeats often have various physiological and pathological implications. DNA repeats are capable of interchanging between different noncanonical and canonical conformations in a dynamic fashion, causing configurational slippage that often leads to repeat expansion associated with neurological diseases. In this report, we used single-molecule spectroscopy together with biophysical analyses to demonstrate the parity-dependent hairpin structural polymorphism of TGGAA repeat DNA. We found that the DNA adopted two configurations depending on the repeat number parity (even or odd). Transitions between these two configurations were also observed for longer repeats. In addition, the ability to modulate this transition was found to be enhanced by divalent ions. Based on the atomic structure, we propose a local seeding model where the kinked GGA motifs in the stem region of TGGAA repeat DNA act as hot spots to facilitate the transition between the two configurations, which may give rise to disease-associated repeat expansion.

PubMed: 28827328
DOI: 10.1073/pnas.1708691114

実験手法

X-RAY DIFFRACTION (2.588 Å)