5GUH

Crystal structure of silkworm PIWI-clade Argonaute Siwi bound to piRNA

5GUH の概要

• エントリーDOI: 10.2210/pdb5guh/pdb
• 分子名称: PIWI, RNA (28-MER), MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: nuclease, rnaseh, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Bombyx mori (Silk moth); 詳細
• 細胞内の位置: Cytoplasm : A8D8P8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 110558.40

構造登録者

Matsumoto, N.,Nishimasu, H.,Ishitani, R.,Nureki, O. (登録日: 2016-08-29, 公開日: 2016-10-19, 最終更新日: 2023-11-08)

主引用文献

Matsumoto, N.,Nishimasu, H.,Sakakibara, K.,Nishida, K.M.,Hirano, T.,Ishitani, R.,Siomi, H.,Siomi, M.C.,Nureki, O.
Crystal Structure of Silkworm PIWI-Clade Argonaute Siwi Bound to piRNA
Cell, 167:484-497.e9, 2016

PubMed Abstract: PIWI-clade Argonaute proteins associate with PIWI-interacting RNAs (piRNAs) and silence transposable elements in animal gonads. Here, we report the crystal structure of a silkworm PIWI-clade Argonaute, Siwi, bound to the endogenous piRNA, at 2.4 Å resolution. Siwi adopts a bilobed architecture consisting of N-PAZ and MID-PIWI lobes, in which the 5' and 3' ends of the bound piRNA are anchored by the MID-PIWI and PAZ domains, respectively. A structural comparison of Siwi with AGO-clade Argonautes reveals notable differences in their nucleic-acid-binding channels, likely reflecting the distinct lengths of their guide RNAs and their mechanistic differences in guide RNA loading and cleavage product release. In addition, the structure reveals that Siwi and prokaryotic, but not eukaryotic, AGO-clade Argonautes share unexpected similarities, such as metal-dependent 5'-phosphate recognition and a potential structural transition during the catalytic-tetrad formation. Overall, this study provides a critical starting point toward a mechanistic understanding of piRNA-mediated transposon silencing.

PubMed: 27693359
DOI: 10.1016/j.cell.2016.09.002

実験手法

X-RAY DIFFRACTION (2.4 Å)