5GU1

Crystal structure of Au(L).CL-apo-E45C/R52C-rHLFr

5GU1 の概要

• エントリーDOI: 10.2210/pdb5gu1/pdb
• 関連するPDBエントリー: 5GU0 5GU2 5GU3
• 分子名称: Ferritin light chain, GOLD ION, CADMIUM ION, ... (6 entities in total)
• 機能のキーワード: iron storage, metal binding protein
• 由来する生物種: Equus caballus (Horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22555.84

構造登録者

Maity, B.,Abe, S.,Ueno, T. (登録日: 2016-08-24, 公開日: 2017-03-29, 最終更新日: 2024-03-20)

主引用文献

Maity, B.,Abe, S.,Ueno, T.
Observation of gold sub-nanocluster nucleation within a crystalline protein cage
Nat Commun, 8:14820-14820, 2017

PubMed Abstract: Protein scaffolds provide unique metal coordination environments that promote biomineralization processes. It is expected that protein scaffolds can be developed to prepare inorganic nanomaterials with important biomedical and material applications. Despite many promising applications, it remains challenging to elucidate the detailed mechanisms of formation of metal nanoparticles in protein environments. In the present work, we describe a crystalline protein cage constructed by crosslinking treatment of a single crystal of apo-ferritin for structural characterization of the formation of sub-nanocluster with reduction reaction. The crystal structure analysis shows the gradual movement of the Au ions towards the centre of the three-fold symmetric channels of the protein cage to form a sub-nanocluster with accompanying significant conformational changes of the amino-acid residues bound to Au ions during the process. These results contribute to our understanding of metal core formation as well as interactions of the metal core with the protein environment.

PubMed: 28300064
DOI: 10.1038/ncomms14820

実験手法

X-RAY DIFFRACTION (2.05 Å)