5GSE

Crystal structure of unusual nucleosome

5GSE の概要

• エントリーDOI: 10.2210/pdb5gse/pdb
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (6 entities in total)
• 機能のキーワード: histone-fold, dna-binding, nucleus, structural protein-dna complex, structural protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus: P68431 P62805 P04908 P06899
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 350035.90

構造登録者

Kato, D.,Osakabe, A.,Arimura, Y.,Park, S.Y.,Kurumizaka, H. (登録日: 2016-08-16, 公開日: 2017-05-03, 最終更新日: 2023-11-08)

主引用文献

Kato, D.,Osakabe, A.,Arimura, Y.,Mizukami, Y.,Horikoshi, N.,Saikusa, K.,Akashi, S.,Nishimura, Y.,Park, S.Y.,Nogami, J.,Maehara, K.,Ohkawa, Y.,Matsumoto, A.,Kono, H.,Inoue, R.,Sugiyama, M.,Kurumizaka, H.
Crystal structure of the overlapping dinucleosome composed of hexasome and octasome
Science, 356:205-208, 2017

PubMed Abstract: Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate "overlapping dinucleosome." Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual "hexasome" nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical "octasome" nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.

PubMed: 28408607
DOI: 10.1126/science.aak9867

実験手法

X-RAY DIFFRACTION (3.14 Å)