5GQQ

Structure of ALG-2/HEBP2 Complex

5GQQ の概要

• エントリーDOI: 10.2210/pdb5gqq/pdb
• 分子名称: Heme-binding protein 2, Programmed cell death protein 6, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: ef hands, complex, calcium ions, apoptosis
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm : Q9Y5Z4; Endoplasmic reticulum membrane ; Peripheral membrane protein : O75340
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 80446.61

構造登録者

Liu, X.,Ma, J.,Zhang, H.,Feng, Y. (登録日: 2016-08-08, 公開日: 2016-11-02, 最終更新日: 2024-03-20)

主引用文献

Ma, J.,Zhang, X.,Feng, Y.,Zhang, H.,Wang, X.,Zheng, Y.,Qiao, W.,Liu, X.
Structural and Functional Study of Apoptosis-linked Gene-2Heme-binding Protein 2 Interactions in HIV-1 Production.
J. Biol. Chem., 291:26670-26685, 2016

PubMed Abstract: In the HIV-1 replication cycle, the endosomal sorting complex required for transport (ESCRT) machinery promotes viral budding and release in the late stages. In this process, the ESCRT proteins, ALIX and TSG101, are recruited through interactions with HIV-1 Gag p6. ALG-2, also known as PDCD6, interacts with both ALIX and TSG101 and bridges ESCRT-III and ESCRT-I. In this study, we show that ALG-2 affects HIV-1 production negatively at both the exogenous and endogenous levels. Through a yeast two-hybrid screen, we identified HEBP2 as the binding partner of ALG-2, and we solved the crystal structure of the ALG-2·HEBP2 complex. The function of ALG-2·HEBP2 complex in HIV-1 replication was further explored. ALG-2 inhibits HIV-1 production by affecting Gag expression and distribution, and HEBP2 might aid this process by tethering ALG-2 in the cytoplasm.

PubMed: 27784779
DOI: 10.1074/jbc.M116.752444

実験手法

X-RAY DIFFRACTION (2.2 Å)