5GQN

Crystal structure of in cellulo Cypovirus Polyhedra mutant with deletion of Gly192-Ala194

5GQN の概要

• エントリーDOI: 10.2210/pdb5gqn/pdb
• 関連するPDBエントリー: 5GQI 5GQJ 5GQK 5GQL 5GQM
• 分子名称: Polyhedrin, CHLORIDE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: in vivo protein crystal, polyhedra, viral protein
• 由来する生物種: Bombyx mori cypovirus 1 (BmCPV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28269.63

構造登録者

Abe, S.,Tabe, H.,Ijiri, H.,Yamashita, K.,Hirata, K.,Mori, H.,Ueno, T. (登録日: 2016-08-07, 公開日: 2017-02-15, 最終更新日: 2024-11-13)

主引用文献

Abe, S.,Tabe, H.,Ijiri, H.,Yamashita, K.,Hirata, K.,Atsumi, K.,Shimoi, T.,Akai, M.,Mori, H.,Kitagawa, S.,Ueno, T.
Crystal Engineering of Self-Assembled Porous Protein Materials in Living Cells
ACS Nano, 11:2410-2419, 2017

PubMed Abstract: Crystalline porous materials have been investigated for development of important applications in molecular storage, separations, and catalysis. The potential of protein crystals is increasing as they become better understood. Protein crystals have been regarded as porous materials because they present highly ordered 3D arrangements of protein molecules with high porosity and wide range of pore sizes. However, it remains difficult to functionalize protein crystals in living cells. Here, we report that polyhedra, a natural crystalline protein assembly of polyhedrin monomer (PhM) produced in insect cells infected by cypovirus, can be engineered to extend porous networks by deleting selected amino acid residues located on the intermolecular contact region of PhM. The adsorption rates and quantities of fluorescent dyes stored within the mutant crystals are increased relative to those of the wild-type polyhedra crystal (WTPhC) under both in vitro and in vivo conditions. These results provide a strategy for designing self-assembled protein materials with applications in molecular recognition and storage of exogenous substances in living cell as well as an entry point for development of bioorthogonal chemistry and in vivo crystal structure analysis.

PubMed: 28094987
DOI: 10.1021/acsnano.6b06099

実験手法

X-RAY DIFFRACTION (1.55 Å)