5GPN

Architecture of mammalian respirasome

これはPDB形式変換不可エントリーです。

5GPN の概要

• エントリーDOI: 10.2210/pdb5gpn/pdb
• EMDBエントリー: 9534
• 分子名称: Cytochrome b-c1 complex subunit 1, mitochondrial, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit 10, ... (73 entities in total)
• 機能のキーワード: respiratory, respirasome, mammalian, electron transport, oxidoreductase
• 由来する生物種: Bos taurus (Bovine); 詳細
• 細胞内の位置: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side: P31800 P23004 F1SRG2; Mitochondrion inner membrane ; Multi-pass membrane protein : P00157 O79874 O79875 Q9TDR1 P00396 P68530; Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side: P00125; Mitochondrion inner membrane: P13271 P00126 P00130 P07552 F1S031 P00423 P00426 P00428 P07471 P04038 P07470 P13183 P00430 P10175 F1STY1 F1SLR1; Mitochondrion inner membrane; Single-pass membrane protein: P13272; Mitochondrion membrane ; Multi- pass membrane protein : O79880 O79881 P56632 O79882; Mitochondrion matrix : F1SIS9; Mitochondrion intermembrane space: P00429
• タンパク質・核酸の鎖数: 84
• 化学式量合計: 1741770.91

構造登録者

Gu, J.,Wu, M.,Guo, R.,Yang, M. (登録日: 2016-08-03, 公開日: 2017-04-05, 最終更新日: 2024-10-09)

主引用文献

Gu, J.,Wu, M.,Guo, R.,Yan, K.,Lei, J.,Gao, N.,Yang, M.
The architecture of the mammalian respirasome.
Nature, 537:639-643, 2016

PubMed Abstract: The respiratory chain complexes I, III and IV (CI, CIII and CIV) are present in the bacterial membrane or the inner mitochondrial membrane and have a role of transferring electrons and establishing the proton gradient for ATP synthesis by complex V. The respiratory chain complexes can assemble into supercomplexes (SCs), but their precise arrangement is unknown. Here we report a 5.4 Å cryo-electron microscopy structure of the major 1.7 megadalton SCI1III2IV1 respirasome purified from porcine heart. The CIII dimer and CIV bind at the same side of the L-shaped CI, with their transmembrane domains essentially aligned to form a transmembrane disk. Compared to free CI, the CI in the respirasome is more compact because of interactions with CIII and CIV. The NDUFA11 and NDUFB9 supernumerary subunits of CI contribute to the oligomerization of CI and CIII. The structure of the respirasome provides information on the precise arrangements of the respiratory chain complexes in mitochondria.

PubMed: 27654917
DOI: 10.1038/nature19359

実験手法

ELECTRON MICROSCOPY (5.4 Å)