5GPE

Crystal structure of the transcription regulator PbrR691 from Ralstonia metallidurans CH34 in complex with Lead(II)

5GPE の概要

• エントリーDOI: 10.2210/pdb5gpe/pdb
• 分子名称: Transcriptional regulator, MerR-family, LEAD (II) ION (3 entities in total)
• 機能のキーワード: pbrr691, merr family, transcription activator, transcription
• 由来する生物種: Cupriavidus metallidurans CH34
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 121324.66

構造登録者

Huang, S.Q.,Chen, W.Z.,Wang, D.,Hu, Q.Y.,Liu, X.C.,Gan, J.H.,Chen, H. (登録日: 2016-08-01, 公開日: 2016-12-28, 最終更新日: 2023-11-08)

主引用文献

Huang, S.Q.,Liu, X.C.,Wang, D.,Chen, W.Z.,Hu, Q.Y.,Wei, T.B.,Zhou, W.Q.,Gan, J.H.,Chen, H.
Structural Basis for the Selective Pb(II) Recognition of Metalloregulatory Protein PbrR691
Inorg Chem, 55:12516-12519, 2016

PubMed Abstract: The transcription regulator PbrR691, one of the MerR family proteins, shows extremely high sensitivity and selectivity toward Pb(II) in Ralstonia metallidurans CH34. Here, we present the crystal structure of PbrR691 in complex with Pb(II) at 2.0 Å resolution. The Pb(II) coordinates with three conserved cysteines and adopts a unique trigonal-pyramidal (hemidirected) geometry. To our knowledge, the PbrR691-Pb(II) structure provides the first three-dimensional visualization of a functional hemidirected lead(II) thiolate coordinate geometry in a protein.

PubMed: 27989185
DOI: 10.1021/acs.inorgchem.6b02397

実験手法

X-RAY DIFFRACTION (2.01 Å)