5GNX

The E171Q mutant structure of Bgl6

5GNX の概要

• エントリーDOI: 10.2210/pdb5gnx/pdb
• 関連するPDBエントリー: 5GNY 5GNZ
• 分子名称: Beta-glucosidase, PROPANOIC ACID, beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: glycosidase, hydrolase
• 由来する生物種: metagenome
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 106372.91

構造登録者

Xie, W.,Pang, P.,Cao, L.C.,Liu, Y.H.,Wang, Z. (登録日: 2016-07-25, 公開日: 2017-04-12, 最終更新日: 2023-11-08)

主引用文献

Pang, P.,Cao, L.C.,Liu, Y.H.,Xie, W.,Wang, Z.
Structures of a glucose-tolerant beta-glucosidase provide insights into its mechanism.
J. Struct. Biol., 198:154-162, 2017

PubMed Abstract: Cellulose can be converted to ethanol via the fermentation of glucose, which is considered as a promising green alternative for transportation fuels. The conversion of cellulose to glucose needs three enzymes, in which β-glucosidase (BGL) plays an essential role. However, BGL is inhibited by its own product glucose, greatly limiting its applications in industry. We previously obtained a novel BGL named Bgl6 with a high glucose tolerance. Further engineering through random mutagenesis produced a triple mutant M3 with improved thermostability. This enzyme shows promising properties for wide applications but the structural basis of the unusual properties of Bgl6 is not clear. In this study, we determined the crystal structures of Bgl6 and variants at high resolution, which provide insights into its glucose-tolerant mechanism and thermostability. Particularly, Bgl6 forms an extra channel that could be used as a secondary binding site for glucose, which may contribute to glucose tolerance. Additionally, the triple mutations could strengthen the hydrophobic interactions within the enzyme and may be responsible for the enhanced thermostability exhibited by M3, which was further confirmed by dynamic light scattering data. Lastly, structural comparison to other orthologs allows us to formulate new strategies on how to improve the catalytic efficiency of Bgl6.

PubMed: 28189793
DOI: 10.1016/j.jsb.2017.02.001

実験手法

X-RAY DIFFRACTION (1.8 Å)