5GNC

Crystal structure of Phytophthora. sojae PSR2

5GNC の概要

• エントリーDOI: 10.2210/pdb5gnc/pdb
• 分子名称: Avh146 (1 entity in total)
• 機能のキーワード: psr2, rnai, unknown function
• 由来する生物種: Phytophthora sojae (Soybean stem and root rot agent)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 68991.92

構造登録者

He, J.Q.,Wu, B.X.,Ma, J.B. (登録日: 2016-07-20, 公開日: 2017-08-16, 最終更新日: 2024-10-30)

主引用文献

He, J.Q.,Ye, W.W.,Choi, D.S.,Wu, B.X.,Zhai, Y.,Guo, B.D.,Duan, S.Y.,Wang, Y.C.,Gan, J.H.,Ma, W.B.,Ma, J.B.
Structural analysis ofPhytophthorasuppressor of RNA silencing 2 (PSR2) reveals a conserved modular fold contributing to virulence.
Proc. Natl. Acad. Sci. U.S.A., 2019

PubMed Abstract: are eukaryotic pathogens that cause enormous losses in agriculture and forestry. Each species encodes hundreds of effector proteins that collectively have essential roles in manipulating host cellular processes and facilitating disease development. Here we report the crystal structure of the effector suppressor of RNA silencing 2 (PSR2). PSR2 produced by the soybean pathogen (PSR2) consists of seven tandem repeat units, including one W-Y motif and six L-W-Y motifs. Each L-W-Y motif forms a highly conserved fold consisting of five α-helices. Adjacent units are connected through stable, directional linkages between an internal loop at the C terminus of one unit and a hydrophobic pocket at the N terminus of the following unit. This unique concatenation results in an overall stick-like structure of PSR2. Genome-wide analyses reveal 293 effectors from five species that have the PSR2-like arrangement, that is, containing a W-Y motif as the "start" unit, various numbers of L-W-Y motifs as the "middle" units, and a degenerate L-W-Y as the "end" unit. Residues involved in the interunit interactions show significant conservation, suggesting that these effectors also use the conserved concatenation mechanism. Furthermore, functional analysis demonstrates differential contributions of individual units to the virulence activity of PSR2. These findings suggest that the L-W-Y fold is a basic structural and functional module that may serve as a "building block" to accelerate effector evolution in .

PubMed: 30926664
DOI: 10.1073/pnas.1819481116

実験手法

X-RAY DIFFRACTION (2.8 Å)