5GN0

Structure of TAZ-TEAD complex

5GN0 の概要

• エントリーDOI: 10.2210/pdb5gn0/pdb
• 分子名称: Transcriptional enhancer factor TEF-3, WW domain-containing transcription regulator protein 1, CITRIC ACID, ... (5 entities in total)
• 機能のキーワード: transcription
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Nucleus: Q62296 Q9EPK5
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 123151.67

構造登録者

Kaan, H.Y.K.,Song, H. (登録日: 2016-07-18, 公開日: 2017-05-31, 最終更新日: 2024-11-20)

主引用文献

Kaan, H.Y.K.,Chan, S.W.,Tan, S.K.J.,Guo, F.,Lim, C.J.,Hong, W.,Song, H.
Crystal structure of TAZ-TEAD complex reveals a distinct interaction mode from that of YAP-TEAD complex
Sci Rep, 7:2035-2035, 2017

PubMed Abstract: The Hippo pathway is a tumor suppressor pathway that is implicated in the regulation of organ size. The pathway has three components: the upstream regulatory factors, the kinase core, and the downstream transcriptional machinery, which consists of YAP, TAZ (transcription co-activators) and TEAD (transcription factor). Formation of YAP/TAZ-TEAD complexes leads to the transcription of growth-promoting genes. Herein, we report the crystal structure of TAZ-TEAD4 complex, which reveals two binding modes. The first is similar to the published YAP-TEAD structure. The second is a unique binding mode, whereby two molecules of TAZ bind to and bridge two molecules of TEAD4. We validated the latter using cross-linking and multi-angle light scattering. Using siRNA, we showed that TAZ knockdown leads to a decrease in TEAD4 dimerization. Lastly, results from luciferase assays, using YAP/TAZ transfected or knockdown cells, give support to the non-redundancy of YAP/TAZ co-activators in regulating gene expression in the Hippo pathway.

PubMed: 28515457
DOI: 10.1038/s41598-017-02219-9

実験手法

X-RAY DIFFRACTION (2.9 Å)