5GMB

Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruiginosa

5GMB の概要

• エントリーDOI: 10.2210/pdb5gmb/pdb
• 関連するPDBエントリー: 5GM8 5GMC
• 分子名称: tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ (2 entities in total)
• 機能のキーワード: pseudomonas aeruginosa, trna methyltransferase, trmj, oxidative stress, trna modification, wobble base, transferase
• 由来する生物種: Pseudomonas aeruginosa
• 細胞内の位置: Cytoplasm : A0A072ZPM2
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18514.09

構造登録者

Jaroensuk, J.,Atichartpongkul, S.,Chionh, Y.H.,Wong, Y.H.,Liew, C.W.,McBee, M.E.,Thongdee, N.,Prestwich, E.G.,DeMott, M.S.,Mongkolsuk, S.,Dedon, P.C.,Lescar, J.,Fuangthong, M. (登録日: 2016-07-13, 公開日: 2016-10-26, 最終更新日: 2023-11-08)

主引用文献

Jaroensuk, J.,Atichartpongkul, S.,Chionh, Y.H.,Wong, Y.H.,Liew, C.W.,McBee, M.E.,Thongdee, N.,Prestwich, E.G.,DeMott, M.S.,Mongkolsuk, S.,Dedon, P.C.,Lescar, J.,Fuangthong, M.
Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruginosa.
Nucleic Acids Res., 44:10834-10848, 2016

PubMed Abstract: Bacteria respond to environmental stresses using a variety of signaling and gene expression pathways, with translational mechanisms being the least well understood. Here, we identified a tRNA methyltransferase in Pseudomonas aeruginosa PA14, trmJ, which confers resistance to oxidative stress. Analysis of tRNA from a trmJ mutant revealed that TrmJ catalyzes formation of Cm, Um, and, unexpectedly, Am. Defined in vitro analyses revealed that tRNA and tRNA are substrates for Cm formation, tRNA, tRNA, tRNA and tRNA for Um, and tRNA for Am. tRNA, previously observed as a TrmJ substrate in Escherichia coli, was not modified by PA14 TrmJ. Position 32 was confirmed as the TrmJ target for Am in tRNA and Um in tRNA by mass spectrometric analysis. Crystal structures of the free catalytic N-terminal domain of TrmJ show a 2-fold symmetrical dimer with an active site located at the interface between the monomers and a flexible basic loop positioned to bind tRNA, with conformational changes upon binding of the SAM-analog sinefungin. The loss of TrmJ rendered PA14 sensitive to HO exposure, with reduced expression of oxyR-recG, katB-ankB, and katE These results reveal that TrmJ is a tRNA:Cm32/Um32/Am32 methyltransferase involved in translational fidelity and the oxidative stress response.

PubMed: 27683218
DOI: 10.1093/nar/gkw870

実験手法

X-RAY DIFFRACTION (1.62 Å)