5GJA

Crystal structure of Arabidopsis thaliana ACO2 in complex with 2-PA

5GJA の概要

• エントリーDOI: 10.2210/pdb5gja/pdb
• 関連するPDBエントリー: 5GJ9
• 分子名称: 1-aminocyclopropane-1-carboxylate oxidase 2, ZINC ION, PYRIDINE-2-CARBOXYLIC ACID, ... (4 entities in total)
• 機能のキーワード: ethylene oxidase inhibitor, oxidoreductase
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 277589.18

構造登録者

Sun, X.Z.,Li, Y.X.,He, W.R.,Ji, C.G.,Xia, P.X.,Wang, Y.C.,Du, S.,Li, H.J.,Raikhel, N.,Xiao, J.Y.,Guo, H.W. (登録日: 2016-06-28, 公開日: 2017-05-10, 最終更新日: 2024-03-20)

主引用文献

Sun, X.,Li, Y.,He, W.,Ji, C.,Xia, P.,Wang, Y.,Du, S.,Li, H.,Raikhel, N.,Xiao, J.,Guo, H.
Pyrazinamide and derivatives block ethylene biosynthesis by inhibiting ACC oxidase.
Nat Commun, 8:15758-15758, 2017

PubMed Abstract: Ethylene is an important phytohormone that promotes the ripening of fruits and senescence of flowers thereby reducing their shelf lives. Specific ethylene biosynthesis inhibitors would help to decrease postharvest loss. Here, we identify pyrazinamide (PZA), a clinical drug used to treat tuberculosis, as an inhibitor of ethylene biosynthesis in Arabidopsis thaliana, using a chemical genetics approach. PZA is converted to pyrazinecarboxylic acid (POA) in plant cells, suppressing the activity of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), the enzyme catalysing the final step of ethylene formation. The crystal structures of Arabidopsis ACO2 in complex with POA or 2-Picolinic Acid (2-PA), a POA-related compound, reveal that POA/2-PA bind at the active site of ACO, preventing the enzyme from interacting with its natural substrates. Our work suggests that PZA and its derivatives may be promising regulators of plant metabolism, in particular ethylene biosynthesis.

PubMed: 28604689
DOI: 10.1038/ncomms15758

実験手法

X-RAY DIFFRACTION (2.1 Å)