5GI0

Crystal structure of RNA editing factor MORF9/RIP9

5GI0 の概要

• エントリーDOI: 10.2210/pdb5gi0/pdb
• 分子名称: Multiple organellar RNA editing factor 9, chloroplastic (2 entities in total)
• 機能のキーワード: rna editing factor, morf9, rip9, rna binding protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• 細胞内の位置: Plastid, chloroplast : Q9LPZ1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15734.68

構造登録者

Yan, J.,Zhang, Q.,Guan, Z.,Zou, T.,Yin, P. (登録日: 2016-06-21, 公開日: 2017-05-10, 最終更新日: 2024-10-16)

主引用文献

Yan, J.,Zhang, Q.,Guan, Z.,Wang, Q.,Li, L.,Ruan, F.,Lin, R.,Zou, T.,Yin, P.
MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing
Nat Plants, 3:17037-17037, 2017

PubMed Abstract: RNA editing is a post-transcriptional process that modifies the genetic information on RNA molecules. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria. The PLS-type pentatricopeptide repeat (PPR) protein and the multiple organellar RNA editing factor (MORF, also known as RNA editing factor interacting protein (RIP)) are two types of key trans-acting factors involved in this process. However, how they cooperate with one another remains unclear. Here, we have characterized the interactions between a designer PLS-type PPR protein (PLS)PPR and MORF9, and found that RNA-binding activity of (PLS)PPR is drastically increased on MORF9 binding. We also determined the crystal structures of (PLS)PPR, MORF9 and the (PLS)PPR-MORF9 complex. MORF9 binding induces significant compressed conformational changes of (PLS)PPR, revealing the molecular mechanisms by which MORF9-bound (PLS)PPR has increased RNA-binding activity. Similarly, increased RNA-binding activity is observed for the natural PLS-type PPR protein, LPA66, in the presence of MORF9. These findings significantly expand our understanding of MORF function in plant organellar RNA editing.

PubMed: 28394309
DOI: 10.1038/nplants.2017.37

実験手法

X-RAY DIFFRACTION (2.044 Å)