5GAF

RNC in complex with SRP

5GAF の概要

• エントリーDOI: 10.2210/pdb5gaf/pdb
• EMDBエントリー: 8002
• 分子名称: SRP 4.5S RNA, 50S ribosomal protein L9, 50S ribosomal protein L10, ... (39 entities in total)
• 機能のキーワード: ribosome, srp, sr
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 1453701.95

構造登録者

Jomaa, A.,Boehringer, D.,Leibundgut, M.,Ban, N. (登録日: 2015-11-25, 公開日: 2016-02-03, 最終更新日: 2024-05-15)

主引用文献

Jomaa, A.,Boehringer, D.,Leibundgut, M.,Ban, N.
Structures of the E. coli translating ribosome with SRP and its receptor and with the translocon
Nat Commun, 7:10471-10471, 2016

PubMed Abstract: Co-translational protein targeting to membranes is a universally conserved process. Central steps include cargo recognition by the signal recognition particle and handover to the Sec translocon. Here we present snapshots of key co-translational-targeting complexes solved by cryo-electron microscopy at near-atomic resolution, establishing the molecular contacts between the Escherichia coli translating ribosome, the signal recognition particle and the translocon. Our results reveal the conformational changes that regulate the latching of the signal sequence, the release of the heterodimeric domains of the signal recognition particle and its receptor, and the handover of the signal sequence to the translocon. We also observe that the signal recognition particle and the translocon insert-specific structural elements into the ribosomal tunnel to remodel it, possibly to sense nascent chains. Our work provides structural evidence for a conformational state of the signal recognition particle and its receptor primed for translocon binding to the ribosome-nascent chain complex.

PubMed: 26804923
DOI: 10.1038/ncomms10471

実験手法

ELECTRON MICROSCOPY (4.3 Å)