5G4U

Association of three two-k-turn units based on Kt-7 3bU,3nU, forming a triangular-shaped structure

5G4U の概要

• エントリーDOI: 10.2210/pdb5g4u/pdb
• 関連するPDBエントリー: 5G4T
• 分子名称: HMKT-7, 50S RIBOSOMAL PROTEIN L7AE (3 entities in total)
• 機能のキーワード: rna-protein complex, rna, kink turn, l7ae, nano crystal packing, nanotechnology, rna/protein
• 由来する生物種: ARCHAEOGLOBUS FULGIDUS; 詳細
• 細胞内の位置: Cytoplasm : O29494
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 118165.87

構造登録者

Huang, L.,Lilley, D.M.J. (登録日: 2016-05-17, 公開日: 2016-09-28, 最終更新日: 2024-01-10)

主引用文献

Huang, L.,Lilley, D.M.J.
A Quasi-Cyclic RNA Nano-Scale Molecular Object Constructed Using Kink Turns.
Nanoscale, 8:15189-, 2016

PubMed Abstract: k-Turns are widespread RNA architectural elements that mediate tertiary interactions. We describe a double-kink-turn motif comprising two inverted k-turns that forms a tight horse-shoe structure that can assemble into a variety of shapes by coaxial association of helical ends. Using X-ray crystallography we show that these assemble with two (dumbell), three (triangle) and four units (square), with or without bound protein, within the crystal lattice. In addition, exchange of a single basepair can almost double the pore radius or shape of a molecular assembly. On the basis of this analysis we synthesized a 114 nt self-complementary RNA containing six k-turns. The crystal structure of this species shows that it forms a quasi-cyclic triangular object. These are randomly disposed about the three-fold axis in the crystal lattice, generating a circular RNA of quasi D3 symmetry with a shape reminiscent of that of a cyclohexane molecule in its chair conformation. This work demonstrates that the k-turn is a powerful building block in the construction of nano-scale molecular objects, and illustrates why k-turns are widely used in natural RNA molecules to organize long-range architecture and mediate tertiary contacts.

PubMed: 27506301
DOI: 10.1039/C6NR05186C

実験手法

X-RAY DIFFRACTION (2.65 Å)