5G2F

Type IV-like competence pilin TTHA1222 from Thermus thermophilus

5G2F の概要

• エントリーDOI: 10.2210/pdb5g2f/pdb
• 関連するPDBエントリー: 5G23 5G24 5G25
• 分子名称: TYPE-IV LIKE PILIN TTHA1222, SULFATE ION (3 entities in total)
• 機能のキーワード: unknown function, natural competence, type iv pilus, dna uptake
• 由来する生物種: THERMUS THERMOPHILUS
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 30682.57

構造登録者

Karuppiah, V.,Derrick, J.P. (登録日: 2016-04-07, 公開日: 2016-09-14, 最終更新日: 2024-11-13)

主引用文献

Karuppiah, V.,Thistlethwaite, A.,Derrick, J.P.
Structures of Type Iv Pilins from Thermus Thermophilus Demonstrate Similarities with Type II Secretion System Pseudopilins
J.Struct.Biol., 196:375-, 2016

PubMed Abstract: Type IV pilins are proteins which form polymers that extend from the surface of the bacterial cell; they are involved in mediating a wide variety of functions, including adhesion, motility and natural competence. Here we describe the determination of the crystal structures of three type IVa pilins proteins from the thermophile Thermus thermophilus. They form part of a cluster of pilus-like proteins within the genome; our results show that one, Tt1222, is very closely related to the main structural type IV pilin, PilA4. The other two, Tt1218 and Tt1219, also adopt canonical pilin-like folds but, interestingly, are most closely related to the structures of the type II secretion system pseudopilins, EpsI/GspI and XcpW/GspJ. GspI and GspJ have been shown to form a complex with another pseudopilin, GspK, and this heterotrimeric complex is known to play a key role in initiating assembly of a pseudopilus which is thought to drive the secretion process. The structural similarity of Tt1218 and Tt1219 to GspI and GspJ suggests that they might work in a similar way, to deliver functions associated with type IV pili in T. thermophilus, such as natural competence.

PubMed: 27612581
DOI: 10.1016/J.JSB.2016.08.006

実験手法

X-RAY DIFFRACTION (1.85 Å)