5G2C

The crystal structure of light-driven chloride pump ClR (T102D) mutant at pH 4.5.

5G2C の概要

• エントリーDOI: 10.2210/pdb5g2c/pdb
• 関連するPDBエントリー: 5G28 5G29 5G2A 5G2D
• 分子名称: CHLORIDE PUMPING RHODOPSIN, OLEIC ACID, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: transport protein
• 由来する生物種: NONLABENS MARINUS S1-08
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33189.44

構造登録者

Kim, K.L.,Kwon, S.K.,Jun, S.H.,Cha, J.S.,Kim, H.Y.,Kim, J.H.,Cho, H.S. (登録日: 2016-04-07, 公開日: 2016-10-19, 最終更新日: 2024-10-23)

主引用文献

Kim, K.,Kwon, S.,Jun, S.,Cha, J.S.,Kim, H.,Lee, W.,Kim, J.F.,Cho, H.
Crystal Structure and Functional Characterization of a Light-Driven Chloride Pump Having an Ntq Motif.
Nat.Commun., 7:12677-, 2016

PubMed Abstract: A novel light-driven chloride-pumping rhodopsin (ClR) containing an 'NTQ motif' in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08(T) determined under two conditions at 2.0 and 1.56 Å resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a '3 omega motif' formed by three non-consecutive aromatic amino acids that is correlated with the B-C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.

PubMed: 27554809
DOI: 10.1038/NCOMMS12677

実験手法

X-RAY DIFFRACTION (2.31 Å)