5G0R

METHYL-COENZYME M REDUCTASE I FROM METHANOTHERMOBACTER MARBURGENSIS EXPOSED TO 3-NITROOXYPROPANOL

5G0R の概要

• エントリーDOI: 10.2210/pdb5g0r/pdb
• 分子名称: METHYL-COENZYME M REDUCTASE I SUBUNIT ALPHA, METHYL-COENZYME M REDUCTASE I SUBUNIT BETA, METHYL-COENZYME M REDUCTASE I SUBUNIT GAMMA, ... (10 entities in total)
• 機能のキーワード: transferase, methyl-coenzymem, methyl-coenzymem reductase exposed to 3-nitrooxypropanol, posttranslational modification, binding sites, catalysis, coenzymes, disulfides, hydrogen, ligands, mesna, metalloporphyrins, methane, methanobacterium, nickel, oxidation-reduction, oxdoreductases, phosphothreonine, thioglycine, 3- nitrooxypropanol, inhibitor, greenhouse gas
• 由来する生物種: METHANOTHERMOBACTER MARBURGENSIS; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 276580.86

構造登録者

Wagner, T. (登録日: 2016-03-22, 公開日: 2016-04-13, 最終更新日: 2024-01-10)

主引用文献

Duin, E.C.,Wagner, T.,Shima, S.,Prakash, D.,Cronin, B.,Yanez-Ruiz, D.R.,Duval, S.,Rumbeli, R.,Stemmler, R.T.,Thauer, R.K.,Kindermann, M.
Mode of Action Uncovered for the Specific Reduction of Methane Emissions from Ruminants by the Small Molecule 3-Nitrooxypropanol.
Proc.Natl.Acad.Sci.USA, 113:6172-, 2016

PubMed Abstract: Ruminants, such as cows, sheep, and goats, predominantly ferment in their rumen plant material to acetate, propionate, butyrate, CO2, and methane. Whereas the short fatty acids are absorbed and metabolized by the animals, the greenhouse gas methane escapes via eructation and breathing of the animals into the atmosphere. Along with the methane, up to 12% of the gross energy content of the feedstock is lost. Therefore, our recent report has raised interest in 3-nitrooxypropanol (3-NOP), which when added to the feed of ruminants in milligram amounts persistently reduces enteric methane emissions from livestock without apparent negative side effects [Hristov AN, et al. (2015) Proc Natl Acad Sci USA 112(34):10663-10668]. We now show with the aid of in silico, in vitro, and in vivo experiments that 3-NOP specifically targets methyl-coenzyme M reductase (MCR). The nickel enzyme, which is only active when its Ni ion is in the +1 oxidation state, catalyzes the methane-forming step in the rumen fermentation. Molecular docking suggested that 3-NOP preferably binds into the active site of MCR in a pose that places its reducible nitrate group in electron transfer distance to Ni(I). With purified MCR, we found that 3-NOP indeed inactivates MCR at micromolar concentrations by oxidation of its active site Ni(I). Concomitantly, the nitrate ester is reduced to nitrite, which also inactivates MCR at micromolar concentrations by oxidation of Ni(I). Using pure cultures, 3-NOP is demonstrated to inhibit growth of methanogenic archaea at concentrations that do not affect the growth of nonmethanogenic bacteria in the rumen.

PubMed: 27140643
DOI: 10.1073/PNAS.1600298113

実験手法

X-RAY DIFFRACTION (1.25 Å)