5G0C

An unusual natural product primary sulfonamide: synthesis, carbonic anhydrase inhibition and protein x-ray structure of Psammaplin C

5G0C の概要

• エントリーDOI: 10.2210/pdb5g0c/pdb
• 関連するPDBエントリー: 5G01 5G03 5G0B
• 分子名称: CARBONIC ANHYDRASE 2, ZINC ION, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: lyase, protein engineering, natural product inhibitor, carbonic anhydrase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29698.56

構造登録者

Mujumdar, P.,Supuran, C.T.,Peat, T.S.,Poulsen, S.A. (登録日: 2016-03-17, 公開日: 2016-05-25, 最終更新日: 2024-01-10)

主引用文献

Mujumdar, P.,Teruya, K.,Tonissen, K.F.,Vullo, D.,Supuran, C.T.,Peat, T.S.,Poulsen, S.
An Unusual Natural Product Primary Sulfonamide: Synthesis, Carbonic Anhydrase Inhibition and Protein X-Ray Structures of Psammaplin C.
J.Med.Chem., 59:5462-, 2016

PubMed Abstract: Psammaplin C is one of only two described natural product primary sulfonamides. Here we report the synthesis of psammaplin C and evaluate the inhibition profile against therapeutically relevant carbonic anhydrase (CA) zinc metalloenzymes. The compound exhibited unprecedented inhibition of an important cancer-associated isozyme, hCA XII, with a Ki of 0.79 nM. The compound also displayed good isoform selectivity for hCA XII over other CAs. We present the first reported protein X-ray crystal structures of psammaplin C in complex with human CAs. We engineered the easily crystallized hCA II enzyme to mimic both the hCA IX and hCA XII binding sites and then utilized protein X-ray crystallography to determine the binding pose of psammaplin C within the hCA II, hCA IX, and hCA XII mimic active sites, all to high resolution. This is the first time a natural product primary sulfonamide inhibitor has been assessed for inhibition and binding to CAs.

PubMed: 27172398
DOI: 10.1021/ACS.JMEDCHEM.6B00443

実験手法

X-RAY DIFFRACTION (1.28 Å)