5G06

Cryo-EM structure of yeast cytoplasmic exosome

5G06 の概要

• エントリーDOI: 10.2210/pdb5g06/pdb
• EMDBエントリー: 3366
• 分子名称: EXOSOME COMPLEX COMPONENT RRP45, EXOSOME COMPLEX EXONUCLEASE DIS3, SUPERKILLER PROTEIN 7, ... (11 entities in total)
• 機能のキーワード: hydrolase, rna decay, exosome, rna quality control
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• 細胞内の位置: Cytoplasm : Q05636 Q08162 Q08491 P46948 P25359 P53256 Q12277 P48240 Q08285 P38792 P53859
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 483231.21

構造登録者

Liu, J.J.,Niu, C.Y.,Wu, Y.,Tan, D.,Wang, Y.,Ye, M.D.,Liu, Y.,Zhao, W.W.,Zhou, K.,Liu, Q.S.,Dai, J.B.,Yang, X.R.,Dong, M.Q.,Huang, N.,Wang, H.W. (登録日: 2016-03-17, 公開日: 2016-06-15, 最終更新日: 2024-11-06)

主引用文献

Liu, J.,Niu, C.,Wu, Y.,Tan, D.,Wang, Y.,Ye, M.,Liu, Y.,Zhao, W.,Zhou, K.,Liu, Q.,Dai, J.,Yang, X.,Dong, M.,Huang, N.,Wang, H.
Cryoem Structure of Yeast Cytoplasmic Exosome Complex.
Cell Res., 26:822-, 2016

PubMed Abstract: The eukaryotic multi-subunit RNA exosome complex plays crucial roles in 3'-to-5' RNA processing and decay. Rrp6 and Ski7 are the major cofactors for the nuclear and cytoplasmic exosomes, respectively. In the cytoplasm, Ski7 helps the exosome to target mRNAs for degradation and turnover via a through-core pathway. However, the interaction between Ski7 and the exosome complex has remained unclear. The transaction of RNA substrates within the exosome is also elusive. In this work, we used single-particle cryo-electron microscopy to solve the structures of the Ski7-exosome complex in RNA-free and RNA-bound forms at resolutions of 4.2 Å and 5.8 Å, respectively. These structures reveal that the N-terminal domain of Ski7 adopts a structural arrangement and interacts with the exosome in a similar fashion to the C-terminal domain of nuclear Rrp6. Further structural analysis of exosomes with RNA substrates harboring 3' overhangs of different length suggests a switch mechanism of RNA-induced exosome activation in the through-core pathway of RNA processing.

PubMed: 27174052
DOI: 10.1038/CR.2016.56

実験手法

ELECTRON MICROSCOPY (4.2 Å)