5FYD

Structural and biochemical insights into 7beta-hydroxysteroid dehydrogenase stereoselectivity

5FYD の概要

• エントリーDOI: 10.2210/pdb5fyd/pdb
• 分子名称: OXIDOREDUCTASE, SHORT CHAIN DEHYDROGENASE/REDUCTASE FAMILY PROTEIN, GLYCEROL (3 entities in total)
• 機能のキーワード: oxidoreductase, short-chain dehydrogenase, steroid, stereoselectivity, dehydrogenase
• 由来する生物種: COLLINSELLA AEROFACIENS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57659.65

構造登録者

Savino, S.,Ferrandi, E.,Forneris, F.,Rovida, S.,Riva, S.,Monti, D.,Mattevi, A. (登録日: 2016-03-07, 公開日: 2016-04-06, 最終更新日: 2024-01-10)

主引用文献

Savino, S.,Ferrandi, E.E.,Forneris, F.,Rovida, S.,Riva, S.,Monti, D.,Mattevi, A.
Structural and Biochemical Insights Into 7Beta-Hydroxysteroid Dehydrogenase Stereoselectivity.
Proteins, 84:859-, 2016

PubMed Abstract: Hydroxysteroid dehydrogenases are of great interest as biocatalysts for transformations involving steroid substrates. They feature a high degree of stereo- and regio-selectivity, acting on a defined atom with a specific configuration of the steroid nucleus. The crystal structure of 7β-hydroxysteroid dehydrogenase from Collinsella aerofaciens reveals a loop gating active-site accessibility, the bases of the specificity for NADP(+) , and the general architecture of the steroid binding site. Comparison with 7α-hydroxysteroid dehydrogenase provides a rationale for the opposite stereoselectivity. The presence of a C-terminal extension reshapes the substrate site of the β-selective enzyme, possibly leading to an inverted orientation of the bound substrate. Proteins 2016; 84:859-865. © 2016 Wiley Periodicals, Inc.

PubMed: 27006087
DOI: 10.1002/PROT.25036

実験手法

X-RAY DIFFRACTION (1.6 Å)