5FVK

Crystal structure of Vps4-Vfa1 complex from S.cerevisiae at 1.66 A resolution.

5FVK の概要

• エントリーDOI: 10.2210/pdb5fvk/pdb
• 関連するPDBエントリー: 5FVL
• 分子名称: VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4, VPS4-ASSOCIATED PROTEIN 1 (3 entities in total)
• 機能のキーワード: hydrolase, vps4, vfa1, mit domain, yeast, atpase
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• 細胞内の位置: Endosome membrane ; Peripheral membrane protein : P52917; Cytoplasm : P40080
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 24089.01

構造登録者

Kojima, R.,Obita, T.,Onoue, K.,Mizuguchi, M. (登録日: 2016-02-09, 公開日: 2016-04-27, 最終更新日: 2024-01-10)

主引用文献

Kojima, R.,Obita, T.,Onoue, K.,Mizuguchi, M.
Structural Fine-Tuning of Mit Interacting Motif 2 (Mim2) and Allosteric Regulation of Escrt-III by Vps4 in Yeast.
J.Mol.Biol., 428:2392-, 2016

PubMed Abstract: The endosomal sorting complex required for transport (ESCRT) facilitates roles in membrane remodeling, such as multivesicular body biogenesis, enveloped virus budding and cell division. In yeast, Vps4 plays a crucial role in intraluminal vesicle formation by disassembling ESCRT proteins. Vps4 is recruited by ESCRT-III proteins to the endosomal membrane through the interaction between the microtubule interacting and trafficking (MIT) domain of Vps4 and the C-terminal MIT-interacting motif (MIM) of ESCRT-III proteins. Here, we have determined the crystal structure of Vps4-MIT in a complex with Vps20, a member of ESCRT-III, and revealed that Vps20 adopts a unique MIM2 conformation. Based on structural comparisons with other known MIM2s, we have refined the consensus sequence of MIM2. We have shown that another ESCRT-III protein, Ist1, binds to Vps4-MIT via its C-terminal MIM1 with higher affinity than Vps2, but lacks MIM2 by surface plasmon resonance. Surprisingly, the Ist1 MIM1 competed with the MIM2 of Vfa1, a regulator of Vps4, for binding to Vps4-MIT, even though these MIMs bind in non-overlapping sites on the MIT. These findings provide insight into the allosteric recognition of MIMs of ESCRT-III by Vps4 and also the regulation of ESCRT machinery at the last step of membrane remodeling.

PubMed: 27075672
DOI: 10.1016/J.JMB.2016.04.007

実験手法

X-RAY DIFFRACTION (1.658 Å)