5FSC

Structure of tectonin 2 from Laccaria bicolor in complex with allyl- alpha_4-methyl-mannoside

5FSC の概要

• エントリーDOI: 10.2210/pdb5fsc/pdb
• 関連するPDBエントリー: 5FSB
• 分子名称: TECTONIN 2, MAGNESIUM ION, prop-2-en-1-yl 4-O-methyl-alpha-D-mannopyranoside, ... (4 entities in total)
• 機能のキーワード: sugar binding protein, lectin, methylated sugar, nematoxic, beta-propeller
• 由来する生物種: LACCARIA BICOLOR
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49783.28

構造登録者

Sommer, R.,Bleuer, S.,Titz, A.,Kunzler, M.,Varrot, A. (登録日: 2016-01-04, 公開日: 2017-03-29, 最終更新日: 2024-01-10)

主引用文献

Sommer, R.,Makshakova, O.N.,Wohlschlager, T.,Hutin, S.,Marsh, M.,Titz, A.,Kunzler, M.,Varrot, A.
Crystal Structures of Fungal Tectonin in Complex with O-Methylated Glycans Suggest Key Role in Innate Immune Defense.
Structure, 26:391-402.e4, 2018

PubMed Abstract: Innate immunity is the first line of defense against pathogens and predators. To initiate a response, it relies on the detection of invaders, where lectin-carbohydrate interactions play a major role. O-Methylated glycans were previously identified as non-self epitopes and conserved targets for defense effector proteins belonging to the tectonin superfamily. Here, we present two crystal structures of Tectonin 2 from the mushroom Laccaria bicolor in complex with methylated ligands, unraveling the molecular basis for this original specificity. Furthermore, they revealed the formation of a ball-shaped tetramer with 24 binding sites distributed at its surface, resembling a small virus capsid. Based on the crystal structures, a methylation recognition motif was identified and found in the sequence of many tectonins from bacteria to human. Our results support a key role of tectonins in innate defense based on a distinctive and conserved type of lectin-glycan interaction.

PubMed: 29398527
DOI: 10.1016/j.str.2018.01.003

実験手法

X-RAY DIFFRACTION (1.95 Å)