5FRS

Structure of the Pds5-Scc1 complex and implications for cohesin function

5FRS の概要

• エントリーDOI: 10.2210/pdb5frs/pdb
• 関連するPDBエントリー: 5FRP 5FRR
• 分子名称: SISTER CHROMATID COHESION PROTEIN PDS5, SISTER CHROMATID COHESION PROTEIN 1 (2 entities in total)
• 機能のキーワード: cohesin, dna replication, sister chromatid cohesion, pds5, smc3, scc1, smc1, wapl, cell cycle
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• 細胞内の位置: Nucleus : Q04264 Q12158
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82629.25

構造登録者

Muir, K.W.,Kschonsak, M.,Li, Y.,Metz, J.,Haering, C.H.,Panne, D. (登録日: 2015-12-22, 公開日: 2016-03-02, 最終更新日: 2024-05-08)

主引用文献

Muir, K.W.,Kschonsak, M.,Li, Y.,Metz, J.,Haering, C.H.,Panne, D.
Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function
Cell Rep., 14:2116-, 2016

PubMed Abstract: Sister chromatid cohesion is a fundamental prerequisite to faithful genome segregation. Cohesion is precisely regulated by accessory factors that modulate the stability with which the cohesin complex embraces chromosomes. One of these factors, Pds5, engages cohesin through Scc1 and is both a facilitator of cohesion, and, conversely also mediates the release of cohesin from chromatin. We present here the crystal structure of a complex between budding yeast Pds5 and Scc1, thus elucidating the molecular basis of Pds5 function. Pds5 forms an elongated HEAT repeat that binds to Scc1 via a conserved surface patch. We demonstrate that the integrity of the Pds5-Scc1 interface is indispensable for the recruitment of Pds5 to cohesin, and that its abrogation results in loss of sister chromatid cohesion and cell viability.

PubMed: 26923589
DOI: 10.1016/J.CELREP.2016.01.078

実験手法

X-RAY DIFFRACTION (4.073 Å)