5FOO

6-phospho-beta-glucosidase

Summary for 5FOO

• Entry DOI: 10.2210/pdb5foo/pdb
• Descriptor: 6-PHOSPHO-BETA-D GLYCOSIDASE, 6-PHOSPHOCYCLOPHELLITOL, 1,2-ETHANEDIOL, ... (7 entities in total)
• Functional Keywords: hydrolase, glycoside hydrolase, cyclophellitol, glucose 6-phosphate
• Biological source: STREPTOCOCCUS PYOGENES
• Total number of polymer chains: 6
• Total formula weight: 334538.79

Authors

Jin, Y.,Kwan, D.H.,Withers, S.G.,Davies, G.J. (deposition date: 2015-11-24, release date: 2016-02-17, Last modification date: 2024-01-10)

Primary citation

Kwan, D.H.,Jin, Y.,Jiang, J.,Chen, H.,Kotzler, M.P.,Overkleeft, H.S.,Davies, G.J.,Withers, S.G.
Chemoenzymatic Synthesis of 6-Phospho-Cyclophellitol as a Novel Probe of 6-Phospho-Beta-Glucosidases.
FEBS Lett., 590:461-, 2016

PubMed Abstract: Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-glucosidase from CAZy family GH1. We further present the three-dimensional structure of the inactivated enzyme, which reveals the constellation of active site residues responsible for the enzyme's specificity and confirms the covalent nature of the inactivation.

PubMed: 26790390
DOI: 10.1002/1873-3468.12059

Experimental method

X-RAY DIFFRACTION (2.1 Å)