5FOO

6-phospho-beta-glucosidase

5FOO の概要

• エントリーDOI: 10.2210/pdb5foo/pdb
• 分子名称: 6-PHOSPHO-BETA-D GLYCOSIDASE, 6-PHOSPHOCYCLOPHELLITOL, 1,2-ETHANEDIOL, ... (7 entities in total)
• 機能のキーワード: hydrolase, glycoside hydrolase, cyclophellitol, glucose 6-phosphate
• 由来する生物種: STREPTOCOCCUS PYOGENES
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 334538.79

構造登録者

Jin, Y.,Kwan, D.H.,Withers, S.G.,Davies, G.J. (登録日: 2015-11-24, 公開日: 2016-02-17, 最終更新日: 2024-01-10)

主引用文献

Kwan, D.H.,Jin, Y.,Jiang, J.,Chen, H.,Kotzler, M.P.,Overkleeft, H.S.,Davies, G.J.,Withers, S.G.
Chemoenzymatic Synthesis of 6-Phospho-Cyclophellitol as a Novel Probe of 6-Phospho-Beta-Glucosidases.
FEBS Lett., 590:461-, 2016

PubMed Abstract: Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-glucosidase from CAZy family GH1. We further present the three-dimensional structure of the inactivated enzyme, which reveals the constellation of active site residues responsible for the enzyme's specificity and confirms the covalent nature of the inactivation.

PubMed: 26790390
DOI: 10.1002/1873-3468.12059

実験手法

X-RAY DIFFRACTION (2.1 Å)