5FNO

Manganese Lipoxygenase

5FNO の概要

• エントリーDOI: 10.2210/pdb5fno/pdb
• 分子名称: MANGANESE LIPOXYGENASE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: oxidoreductase, oxidase, lipoxygenase, magnaporthe oryzae
• 由来する生物種: MAGNAPORTHE ORYZAE (RICE BLAST FUNGUS)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 135962.67

構造登録者

Wennman, A.,Karkehabadi, S.,Oliw, E.H.,Chen, Y. (登録日: 2015-11-16, 公開日: 2016-01-27, 最終更新日: 2025-10-01)

主引用文献

Wennman, A.,Oliw, E.H.,Karkehabadi, S.,Chen, Y.
Crystal Structure of Manganese Lipoxygenase of the Rice Blast Fungus Magnaporthe Oryzae
J.Biol.Chem., 291:8130-, 2016

PubMed Abstract: Lipoxygenases (LOX) are non-heme metal enzymes, which oxidize polyunsaturated fatty acids to hydroperoxides. All LOX belong to the same gene family, and they are widely distributed. LOX of animals, plants, and prokaryotes contain iron as the catalytic metal, whereas fungi express LOX with iron or with manganese. Little is known about metal selection by LOX and the adjustment of the redox potentials of their protein-bound catalytic metals. Thirteen three-dimensional structures of animal, plant, and prokaryotic FeLOX are available, but none of MnLOX. The MnLOX of the most important plant pathogen, the rice blast fungusMagnaporthe oryzae(Mo), was expressed inPichia pastoris.Mo-MnLOX was deglycosylated, purified to homogeneity, and subjected to crystal screening and x-ray diffraction. The structure was solved by sulfur and manganese single wavelength anomalous dispersion to a resolution of 2.0 Å. The manganese coordinating sphere is similar to iron ligands of coral 8R-LOX and soybean LOX-1 but is not overlapping. The Asn-473 is positioned on a short loop (Asn-Gln-Gly-Glu-Pro) instead of an α-helix and forms hydrogen bonds with Gln-281. Comparison with FeLOX suggests that Phe-332 and Phe-525 might contribute to the unique suprafacial hydrogen abstraction and oxygenation mechanism of Mo-MnLOX by controlling oxygen access to the pentadiene radical. Modeling suggests that Arg-525 is positioned close to Arg-182 of 8R-LOX, and both residues likely tether the carboxylate group of the substrate. An oxygen channel could not be identified. We conclude that Mo-MnLOX illustrates a partly unique variation of the structural theme of FeLOX.

PubMed: 26783260
DOI: 10.1074/JBC.M115.707380

実験手法

X-RAY DIFFRACTION (2.038 Å)