5FM9

human Notch 1, EGF 4-7

5FM9 の概要

• エントリーDOI: 10.2210/pdb5fm9/pdb
• 関連するPDBエントリー: 5FMA
• 分子名称: NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1, CALCIUM ION (3 entities in total)
• 機能のキーワード: transcription, transmembrane, developmental, protein, notch signaling pathway, differentiation, phosphorylation, egf- like domain, regulation, receptor, activator, ank repeat, signalling, glycoprotein, extracellular, egf, notch, jagged, membrane
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cell membrane ; Single-pass type I membrane protein . Notch 1 intracellular domain: Nucleus : P46531
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16722.54

構造登録者

Weisshuhn, P.C.,Sheppard, D.,Taylor, P.,Whiteman, P.,Lea, S.M.,Handford, P.A.,Redfield, C. (登録日: 2015-11-02, 公開日: 2016-04-20, 最終更新日: 2024-01-10)

主引用文献

Weisshuhn, P.C.,Sheppard, D.,Taylor, P.,Whiteman, P.,Lea, S.M.,Handford, P.A.,Redfield, C.
Non-Linear and Flexible Regions of the Human Notch1 Extracellular Domain Revealed by High-Resolution Structural Studies.
Structure, 24:555-, 2016

PubMed Abstract: The Notch receptor is a key component of a core metazoan signaling pathway activated by Delta/Serrate/Lag-2 ligands expressed on an adjacent cell. This results in a short-range signal with profound effects on cell-fate determination, cell proliferation, and cell death. Key to understanding receptor function is structural knowledge of the large extracellular portion of Notch which contains multiple repeats of epidermal growth factor (EGF)-like domains. Here we investigate the EGF4-13 region of human Notch1 (hN1) using a multidisciplinary approach. Ca(2+)-binding measurements, X-ray crystallography, {(1)H}-(15)N heteronuclear nuclear Overhauser effects, and residual dipolar couplings support a non-linear organization for the EGF4-13 region with a rigid, bent conformation for EGF4-7 and a single flexible linkage between EGF9 and EGF10. These data allow us to construct an informed model for EGF10-13 which, in conjunction with comparative binding studies, demonstrates that EGF10 has an important role in determining Notch receptor sensitivity to Dll-4.

PubMed: 26996961
DOI: 10.1016/J.STR.2016.02.010

実験手法

X-RAY DIFFRACTION (2.92 Å)