5FHK

Regulatory domain of AphB in Vibrio vulnificus

5FHK の概要

• エントリーDOI: 10.2210/pdb5fhk/pdb
• 分子名称: LysR family transcriptional regulator (2 entities in total)
• 機能のキーワード: aphb, lysr-type, transcriptional regulator, tcpph activation, transcription
• 由来する生物種: Vibrio vulnificus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 140417.88

構造登録者

Song, S.,Ha, N.-C. (登録日: 2015-12-22, 公開日: 2017-01-11, 最終更新日: 2023-11-08)

主引用文献

Park, N.,Song, S.,Choi, G.,Jang, K.K.,Jo, I.,Choi, S.H.,Ha, N.-C.
Crystal Structure of the Regulatory Domain of AphB from Vibrio vulnificus, a Virulence Gene Regulator
Mol. Cells, 40:299-306, 2017

PubMed Abstract: The transcriptional activator AphB has been implicated in acid resistance and pathogenesis in the food borne pathogens and . To date, the full-length AphB crystal structure of has been determined and characterized by a tetrameric assembly of AphB consisting of a DNA binding domain and a regulatory domain (RD). Although acidic pH and low oxygen tension might be involved in the activation of AphB, it remains unknown which ligand or stimulus activates AphB at the molecular level. In this study, we determine the crystal structure of the AphB RD from under aerobic conditions without modification at the conserved cysteine residue of the RD, even in the presence of the oxidizing agent cumene hydroperoxide. A cysteine to serine amino acid residue mutant RD protein further confirmed that the cysteine residue is not involved in sensing oxidative stress in vitro. Interestingly, an unidentified small molecule was observed in the inter-subdomain cavity in the RD when the crystal was incubated with cumene hydroperoxide molecules, suggesting a new ligand-binding site. In addition, we confirmed the role of AphB in acid tolerance by observing an -dependent increase in transcript level when was exposed to acidic pH. Our study contributes to the understanding of the AphB molecular mechanism in the process of recognizing the host environment.

PubMed: 28427249
DOI: 10.14348/molcells.2017.0015

実験手法

X-RAY DIFFRACTION (1.905 Å)