5FHI

Crystallographic structure of PsoE without Co

5FHI の概要

• エントリーDOI: 10.2210/pdb5fhi/pdb
• 分子名称: Glutathione S-transferase, putative, GLUTATHIONE (3 entities in total)
• 機能のキーワード: gst, transferase
• 由来する生物種: Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28022.93

構造登録者

Hara, K.,Hashimoto, H.,Yamamoto, T.,Tsunematsu, Y.,Watanabe, K. (登録日: 2015-12-22, 公開日: 2016-04-20, 最終更新日: 2024-03-20)

主引用文献

Yamamoto, T.,Tsunematsu, Y.,Hara, K.,Suzuki, T.,Kishimoto, S.,Kawagishi, H.,Noguchi, H.,Hashimoto, H.,Tang, Y.,Hotta, K.,Watanabe, K.
Oxidative trans to cis Isomerization of Olefins in Polyketide Biosynthesis.
Angew. Chem. Int. Ed. Engl., 55:6207-6210, 2016

PubMed Abstract: Geometric isomerization can expand the scope of biological activities of natural products. The observed chemical diversity among the pseurotin-type fungal secondary metabolites is in part generated by a trans to cis isomerization of an olefin. In vitro characterizations of pseurotin biosynthetic enzymes revealed that the glutathione S-transferase PsoE requires participation of the bifunctional C-methyltransferase/epoxidase PsoF to complete the trans to cis isomerization of the pathway intermediate presynerazol. The crystal structure of the PsoE/glutathione/presynerazol complex indicated stereospecific glutathione-presynerazol conjugate formation is the principal function of PsoE. Moreover, PsoF was identified to have an additional, unexpected oxidative isomerase activity, thus making it a trifunctional enzyme which is key to the complexity generation in pseurotin biosynthesis. Through the study, we identified a novel mechanism of accomplishing a seemingly simple trans to cis isomerization reaction.

PubMed: 27072782
DOI: 10.1002/anie.201600940

実験手法

X-RAY DIFFRACTION (2.41 Å)