5FFM

Yellow fever virus helicase

「1YMF」から置き換えられました

5FFM の概要

• エントリーDOI: 10.2210/pdb5ffm/pdb
• 分子名称: Serine protease NS3 (2 entities in total)
• 機能のキーワード: dead helicase rna triphosphatase, hydrolase
• 由来する生物種: Yellow fever virus (strain 17D vaccine) (YFV)
• 細胞内の位置: Capsid protein C: Virion . Peptide pr: Secreted. Small envelope protein M: Virion membrane ; Multi-pass membrane protein . Envelope protein E: Virion membrane ; Multi-pass membrane protein . Non-structural protein 1: Secreted. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 2A: Host endoplasmic reticulum membrane ; Multi- pass membrane protein . Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Multi- pass membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi- pass membrane protein . RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side : P03314
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50010.09

構造登録者

Smith, J.L. (登録日: 2015-12-18, 公開日: 2015-12-30, 最終更新日: 2024-10-30)

主引用文献

Wu, J.,Bera, A.K.,Kuhn, R.J.,Smith, J.L.
Structure of the Flavivirus helicase: implications for catalytic activity, protein interactions, and proteolytic processing.
J. Virol., 79:10268-10277, 2005

PubMed Abstract: Yellow fever virus (YFV), a member of the Flavivirus genus, has a plus-sense RNA genome encoding a single polyprotein. Viral protein NS3 includes a protease and a helicase that are essential to virus replication and to RNA capping. The 1.8-A crystal structure of the helicase region of the YFV NS3 protein includes residues 187 to 623. Two familiar helicase domains bind nucleotide in a triphosphate pocket without base recognition, providing a site for nonspecific hydrolysis of nucleoside triphosphates and RNA triphosphate. The third, C-terminal domain has a unique structure and is proposed to function in RNA and protein recognition. The organization of the three domains indicates that cleavage of the viral polyprotein NS3-NS4A junction occurs in trans.

PubMed: 16051820

実験手法

X-RAY DIFFRACTION (2.6 Å)