5FFL

Crystal structure of mouse CD300lf at 1.6 Angstroms resolution.

5FFL の概要

• エントリーDOI: 10.2210/pdb5ffl/pdb
• 分子名称: CD300 antigen-like family member F, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: cd300lf, cd300f, clm-1, clm1, irem-1, irem1, igsf13, lmir3, nkir, inhibitory receptor, ig superfamily, cd300 molecule-like family member f, immunoglobulin superfamily member, cell surface receptor, immune system
• 由来する生物種: Mus musculus
• 細胞内の位置: Cell membrane ; Single-pass type I membrane protein : Q6SJQ7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13284.13

構造登録者

Nelson, C.A.,Fremont, D.H. (登録日: 2015-12-18, 公開日: 2016-08-31, 最終更新日: 2024-11-13)

主引用文献

Orchard, R.C.,Wilen, C.B.,Doench, J.G.,Baldridge, M.T.,McCune, B.T.,Lee, Y.C.,Lee, S.,Pruett-Miller, S.M.,Nelson, C.A.,Fremont, D.H.,Virgin, H.W.
Discovery of a proteinaceous cellular receptor for a norovirus.
Science, 353:933-936, 2016

PubMed Abstract: Noroviruses (NoVs) are a leading cause of gastroenteritis globally, yet the host factors required for NoV infection are poorly understood. We identified host molecules that are essential for murine NoV (MNoV)-induced cell death, including CD300lf as a proteinaceous receptor. We found that CD300lf is essential for MNoV binding and replication in cell lines and primary cells. Additionally, Cd300lf(-/-) mice are resistant to MNoV infection. Expression of CD300lf in human cells breaks the species barrier that would otherwise restrict MNoV replication. The crystal structure of the CD300lf ectodomain reveals a potential ligand-binding cleft composed of residues that are critical for MNoV infection. Therefore, the presence of a proteinaceous receptor is the primary determinant of MNoV species tropism, whereas other components of cellular machinery required for NoV replication are conserved between humans and mice.

PubMed: 27540007
DOI: 10.1126/science.aaf1220

実験手法

X-RAY DIFFRACTION (1.602 Å)