5FD0

Streptomyces plicatus N-acetyl-beta-hexosaminidase in complex with NAGlucal

5FD0 の概要

• エントリーDOI: 10.2210/pdb5fd0/pdb
• 関連するPDBエントリー: 1HP4 1HP5 1JAK 1M01 5FCZ
• 分子名称: B-N-acetylhexosaminidase, 2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: family 20 glycoside hydrolase, sphex, gh20, nag-glucal, hexosaminidase, glycosidase inhibitor, hydrolase
• 由来する生物種: Streptomyces plicatus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56916.43

構造登録者

Vadlamani, G.,Mark, B.L. (登録日: 2015-12-15, 公開日: 2016-10-26, 最終更新日: 2023-09-27)

主引用文献

Santana, A.G.,Vadlamani, G.,Mark, B.L.,Withers, S.G.
N-Acetyl glycals are tight-binding and environmentally insensitive inhibitors of hexosaminidases.
Chem.Commun.(Camb.), 52:7943-7946, 2016

PubMed Abstract: Mono-, di- and trisaccharide derivatives of 1,2-unsaturated N-acetyl-d-glucal have been synthesized and shown to function as tight-binding inhibitors/slow substrates of representative hexosaminidases. Turnover is slow and not observed in the thioamide analogue, allowing determination of the 3-dimensional structure of the complex. Inhibition is insensitive to pH and to mutation of key catalytic residues, consistent with the uncharged character of the inhibitor. These properties could render this inhibitor class less prone to development of resistance.

PubMed: 27253678
DOI: 10.1039/c6cc02520j

実験手法

X-RAY DIFFRACTION (2 Å)