5FB6

Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering

5FB6 の概要

• エントリーDOI: 10.2210/pdb5fb6/pdb
• 関連するPDBエントリー: 9INS
• 分子名称: Insulin Chain A, Insulin Chain B (3 entities in total)
• 機能のキーワード: cubic insulin, hormone
• 由来する生物種: Sus scrofa (Pig); 詳細
• 細胞内の位置: Secreted: P01315 P01315
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5787.63

構造登録者

Roedig, P.,Duman, R.,Sanchez-Weatherby, J.,Vartiainen, I.,Burkhardt, A.,Warmer, M.,David, C.,Wagner, A.,Meents, A. (登録日: 2015-12-14, 公開日: 2016-06-15, 最終更新日: 2024-11-20)

主引用文献

Roedig, P.,Duman, R.,Sanchez-Weatherby, J.,Vartiainen, I.,Burkhardt, A.,Warmer, M.,David, C.,Wagner, A.,Meents, A.
Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering.
J.Appl.Crystallogr., 49:968-975, 2016

PubMed Abstract: Recent success at X-ray free-electron lasers has led to serial crystallography experiments staging a comeback at synchrotron sources as well. With crystal lifetimes typically in the millisecond range and the latest-generation detector technologies with high framing rates up to 1 kHz, fast sample exchange has become the bottleneck for such experiments. A micro-patterned chip has been developed from single-crystalline silicon, which acts as a sample holder for up to several thousand microcrystals at a very low background level. The crystals can be easily loaded onto the chip and excess mother liquor can be efficiently removed. Dehydration of the crystals is prevented by keeping them in a stream of humidified air during data collection. Further sealing of the sample holder, for example with Kapton, is not required. Room-temperature data collection from insulin crystals loaded onto the chip proves the applicability of the chip for macromolecular crystallography. Subsequent structure refinements reveal no radiation-damage-induced structural changes for insulin crystals up to a dose of 565.6 kGy, even though the total diffraction power of the crystals has on average decreased to 19.1% of its initial value for the same dose. A decay of the diffracting power by half is observed for a dose of = 147.5 ± 19.1 kGy, which is about 1/300 of the dose before crystals show a similar decay at cryogenic temperatures.

PubMed: 27275143
DOI: 10.1107/S1600576716006348

実験手法

X-RAY DIFFRACTION (1.901 Å)